miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8725 3' -53.6 NC_002331.1 + 66317 0.66 0.97646
Target:  5'- gGUUUGAGUgauGCCG-CaGUAAGGGaCGCa -3'
miRNA:   3'- -UAAGCUCGag-CGGCuG-CAUUUCC-GCG- -5'
8725 3' -53.6 NC_002331.1 + 152115 0.66 0.97646
Target:  5'- uUUUGccaaGGCUgaCGCUGGCGguccAGGCGCa -3'
miRNA:   3'- uAAGC----UCGA--GCGGCUGCauu-UCCGCG- -5'
8725 3' -53.6 NC_002331.1 + 72117 0.66 0.97646
Target:  5'- -gUCGA-CUCGCCGAU----AGGCGg -3'
miRNA:   3'- uaAGCUcGAGCGGCUGcauuUCCGCg -5'
8725 3' -53.6 NC_002331.1 + 144484 0.66 0.973622
Target:  5'- -cUCGAGUaucgucaauaguaUCGCUGACGguuAGGCu- -3'
miRNA:   3'- uaAGCUCG-------------AGCGGCUGCauuUCCGcg -5'
8725 3' -53.6 NC_002331.1 + 11458 0.67 0.95794
Target:  5'- -gUCG-GCUCGCUGAgGUAAAuuauGGuCGUg -3'
miRNA:   3'- uaAGCuCGAGCGGCUgCAUUU----CC-GCG- -5'
8725 3' -53.6 NC_002331.1 + 152629 0.68 0.926031
Target:  5'- -gUCGcGUUCcgGCCGGCGUuguGGUGCa -3'
miRNA:   3'- uaAGCuCGAG--CGGCUGCAuuuCCGCG- -5'
8725 3' -53.6 NC_002331.1 + 126144 0.7 0.860196
Target:  5'- gGUUUGA-CUUGuuGACGUAGuGGUGCg -3'
miRNA:   3'- -UAAGCUcGAGCggCUGCAUUuCCGCG- -5'
8725 3' -53.6 NC_002331.1 + 51062 0.7 0.844366
Target:  5'- uGUUCGgcuguguaAGaCUCGCCGGCGUugGAGuGGCGUc -3'
miRNA:   3'- -UAAGC--------UC-GAGCGGCUGCA--UUU-CCGCG- -5'
8725 3' -53.6 NC_002331.1 + 156527 0.72 0.754755
Target:  5'- --gCGAuGCUgaCGUCGGCGUAGuGGCGCu -3'
miRNA:   3'- uaaGCU-CGA--GCGGCUGCAUUuCCGCG- -5'
8725 3' -53.6 NC_002331.1 + 65487 0.73 0.712317
Target:  5'- aGUUCGGGCUCGUaCG-UGUAAaacuggcaaucauaGGGCGCa -3'
miRNA:   3'- -UAAGCUCGAGCG-GCuGCAUU--------------UCCGCG- -5'
8725 3' -53.6 NC_002331.1 + 59282 1.09 0.005602
Target:  5'- cAUUCGAGCUCGCCGACGUAAAGGCGCc -3'
miRNA:   3'- -UAAGCUCGAGCGGCUGCAUUUCCGCG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.