miRNA: 3'- uaaaUGGGUCUGUGGg----CaGCAU-UUGC- -5'
miRNA: 3'- uaaaUGG--GUCUGUGGGCaGCA-UUUGC- -5'
miRNA: 3'- uaaAUGGGU-CUGUG--GGCAGCa--UUUGC- -5'
miRNA: 3'- uaaaUGGGUcUGUGGGCAGCAUUUGc -5'
miRNA: 3'- -UAAAUGGGUCUGUGGGCAGCAUUUGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8726 | 5' | -49.1 | NC_002331.1 | + | 45398 | 0.66 | 0.999113 |
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Target: 5'- ---aAUCCAGACACCaaagaGcCGUAcGACGa -3' miRNA: 3'- uaaaUGGGUCUGUGGg----CaGCAU-UUGC- -5' |
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| 8726 | 5' | -49.1 | NC_002331.1 | + | 117865 | 0.69 | 0.990824 |
|
Target: 5'- ---aACCaaCGGGCGCCCGcCGUcAAACGa -3' miRNA: 3'- uaaaUGG--GUCUGUGGGCaGCA-UUUGC- -5' |
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| 8726 | 5' | -49.1 | NC_002331.1 | + | 43432 | 0.7 | 0.980651 |
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Target: 5'- --cUGCCCAgGACACgaCCGUCGacgAGGCGc -3' miRNA: 3'- uaaAUGGGU-CUGUG--GGCAGCa--UUUGC- -5' |
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| 8726 | 5' | -49.1 | NC_002331.1 | + | 8153 | 0.79 | 0.680268 |
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Target: 5'- ---aGCCCAcACAUCCGUCGUAAACc -3' miRNA: 3'- uaaaUGGGUcUGUGGGCAGCAUUUGc -5' |
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| 8726 | 5' | -49.1 | NC_002331.1 | + | 66635 | 1.07 | 0.020418 |
|
Target: 5'- cAUUUACCCAGACACCCGUCGUAAACGu -3' miRNA: 3'- -UAAAUGGGUCUGUGGGCAGCAUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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