miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8727 5' -48.5 NC_002331.1 + 150464 0.68 0.996845
Target:  5'- gUCGGAUGGUUGUccaaccauuGAG-AGUGGuaAUCu -3'
miRNA:   3'- aAGCCUACCAGCAu--------UUCuUCACC--UAG- -5'
8727 5' -48.5 NC_002331.1 + 125623 0.68 0.996845
Target:  5'- -aUGGAauGUgGUAGAGAGGUGGAa- -3'
miRNA:   3'- aaGCCUacCAgCAUUUCUUCACCUag -5'
8727 5' -48.5 NC_002331.1 + 68086 1.09 0.016282
Target:  5'- uUUCGGAUGGUCGUAAAGAAGUGGAUCa -3'
miRNA:   3'- -AAGCCUACCAGCAUUUCUUCACCUAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.