Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8728 | 3' | -40.5 | NC_002331.1 | + | 146263 | 0.76 | 0.999797 |
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Target: 5'- -gGUAAAauUUUUAAUGUCGACCCa--- -3' miRNA: 3'- ggUAUUU--AAAAUUACAGCUGGGguuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 69033 | 0.74 | 0.999974 |
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Target: 5'- aCAcuuuUUUUAAUGUCGAUCCCGu- -3' miRNA: 3'- gGUauuuAAAAUUACAGCUGGGGUuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 171571 | 0.73 | 0.999994 |
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Target: 5'- gUCAaAAAUUUUAAUGUCGAucuuuucgccUCCCGGAg -3' miRNA: 3'- -GGUaUUUAAAAUUACAGCU----------GGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 111680 | 1.11 | 0.093469 |
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Target: 5'- gCCAUAAAUUUUAAUGUCGACCCCAAAg -3' miRNA: 3'- -GGUAUUUAAAAUUACAGCUGGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 46297 | 0.72 | 0.999998 |
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Target: 5'- uCUggAAAUUUUAAUGUCGAUUUCGGAa -3' miRNA: 3'- -GGuaUUUAAAAUUACAGCUGGGGUUU- -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 162612 | 0.71 | 1 |
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Target: 5'- ---aAAAUUUUAAUGUCGAUUUCGAu -3' miRNA: 3'- gguaUUUAAAAUUACAGCUGGGGUUu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 84311 | 0.7 | 1 |
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Target: 5'- --------aUUAGUGUCGACCaCCGAu -3' miRNA: 3'- gguauuuaaAAUUACAGCUGG-GGUUu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 46708 | 0.66 | 1 |
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Target: 5'- aCAcuuuUUUUAAUGUCGACCUUu-- -3' miRNA: 3'- gGUauuuAAAAUUACAGCUGGGGuuu -5' |
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| 8728 | 3' | -40.5 | NC_002331.1 | + | 19652 | 0.66 | 1 |
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Target: 5'- ---cGGGUUUUAAUaUCGACCUCAc- -3' miRNA: 3'- gguaUUUAAAAUUAcAGCUGGGGUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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