Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8729 | 3' | -53.7 | NC_002331.1 | + | 168988 | 0.66 | 0.967696 |
Target: 5'- cUAGCGCCGGU-GAGUgaagauuugaacguGUGUUCGUCGg -3' miRNA: 3'- uGUCGUGGUCGuCUCG--------------UAUAGGCAGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 2135 | 0.66 | 0.965438 |
Target: 5'- aGCAGCAgCAGCAGcAGCAgcaaCGaCAa -3' miRNA: 3'- -UGUCGUgGUCGUC-UCGUauagGCaGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 172671 | 0.66 | 0.962023 |
Target: 5'- cGCA-CACCAGCAGAGU-UAUUCGa-- -3' miRNA: 3'- -UGUcGUGGUCGUCUCGuAUAGGCagu -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 153257 | 0.67 | 0.931511 |
Target: 5'- -gAGCACgAGCAcGAGUGUGUgCCGUUc -3' miRNA: 3'- ugUCGUGgUCGU-CUCGUAUA-GGCAGu -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 41354 | 0.68 | 0.90866 |
Target: 5'- gGCAGCGCgGGCAGuuGCGguaaAUCCGgggugCAg -3' miRNA: 3'- -UGUCGUGgUCGUCu-CGUa---UAGGCa----GU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 130398 | 0.68 | 0.908038 |
Target: 5'- aACAGCACgacaaagUGGCAcGAcCGUGUCCGUCGg -3' miRNA: 3'- -UGUCGUG-------GUCGU-CUcGUAUAGGCAGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 153401 | 0.69 | 0.867108 |
Target: 5'- aGCgAGUACCGGCAGAGU----UCGUCGg -3' miRNA: 3'- -UG-UCGUGGUCGUCUCGuauaGGCAGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 152371 | 0.69 | 0.867108 |
Target: 5'- cCGGUAUUAGCGGAGCGgucuccugUAUCCGgCAg -3' miRNA: 3'- uGUCGUGGUCGUCUCGU--------AUAGGCaGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 65452 | 0.72 | 0.752204 |
Target: 5'- gGCGGagaagcgucCACCGGUGGAGCGUAUUCGUgCAc -3' miRNA: 3'- -UGUC---------GUGGUCGUCUCGUAUAGGCA-GU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 94837 | 0.74 | 0.629248 |
Target: 5'- ---aUugCAGCAGAGCAUccacGUCCGUCGa -3' miRNA: 3'- ugucGugGUCGUCUCGUA----UAGGCAGU- -5' |
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8729 | 3' | -53.7 | NC_002331.1 | + | 112162 | 1.09 | 0.004887 |
Target: 5'- aACAGCACCAGCAGAGCAUAUCCGUCAa -3' miRNA: 3'- -UGUCGUGGUCGUCUCGUAUAGGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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