Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8730 | 3' | -44.7 | NC_002331.1 | + | 52909 | 0.67 | 0.999994 |
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Target: 5'- uGUCGAccuuugcGUCuuGAUGGUUUGGGuCGCGu -3' miRNA: 3'- -UAGCUa------CAGuuUUGCUAAAUCC-GUGC- -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 97309 | 0.68 | 0.999984 |
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Target: 5'- -aCGGUGUCGu--CGAUUaUAGGCAa- -3' miRNA: 3'- uaGCUACAGUuuuGCUAA-AUCCGUgc -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 89341 | 0.68 | 0.999966 |
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Target: 5'- cGUUGcgGUCGAAgcgcACGAUUgcgaauucguuuuuUGGGCACa -3' miRNA: 3'- -UAGCuaCAGUUU----UGCUAA--------------AUCCGUGc -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 62195 | 0.69 | 0.999924 |
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Target: 5'- cGUgGGguugGUCAAAAgGGUUUAGGUGCu -3' miRNA: 3'- -UAgCUa---CAGUUUUgCUAAAUCCGUGc -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 137218 | 0.69 | 0.9999 |
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Target: 5'- aAUUuuUGUCAGAGCGAgcgcugUUuGGCACGa -3' miRNA: 3'- -UAGcuACAGUUUUGCUa-----AAuCCGUGC- -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 133268 | 0.72 | 0.998381 |
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Target: 5'- -aCGAUGUCGAcACGGUguccgagcGGGCGCu -3' miRNA: 3'- uaGCUACAGUUuUGCUAaa------UCCGUGc -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 25246 | 0.72 | 0.998043 |
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Target: 5'- cAUCGGUGUCAGuuCG-UUUAGGgACu -3' miRNA: 3'- -UAGCUACAGUUuuGCuAAAUCCgUGc -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 160747 | 0.73 | 0.99604 |
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Target: 5'- cUCGAcGUCAAAACGGUUguGGUAUGu -3' miRNA: 3'- uAGCUaCAGUUUUGCUAAauCCGUGC- -5' |
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| 8730 | 3' | -44.7 | NC_002331.1 | + | 149518 | 1.08 | 0.047334 |
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Target: 5'- gAUCGAUGUCAAAACGAUUUAGGCACGu -3' miRNA: 3'- -UAGCUACAGUUUUGCUAAAUCCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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