Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8732 | 5' | -48.6 | NC_002331.1 | + | 25030 | 0.66 | 0.999319 |
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Target: 5'- uGUUUGGUaaaaGAACGCGUAccGCaCUGCACAUg -3' miRNA: 3'- -CAAAUCG----CUUGUGUAU--CG-GGUGUGUA- -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 161771 | 0.66 | 0.999319 |
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Target: 5'- ---aAGCGAGCACcaguUGGCCaACugGUa -3' miRNA: 3'- caaaUCGCUUGUGu---AUCGGgUGugUA- -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 49675 | 0.66 | 0.999158 |
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Target: 5'- --aUAGUGu-CACGUAGUCCGgGCGUu -3' miRNA: 3'- caaAUCGCuuGUGUAUCGGGUgUGUA- -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 88277 | 0.67 | 0.998143 |
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Target: 5'- ---gGGUGGGCACGUGuUCCACACu- -3' miRNA: 3'- caaaUCGCUUGUGUAUcGGGUGUGua -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 102518 | 0.69 | 0.989331 |
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Target: 5'- --aUGGCGAccaaACAgAUAGCCCuGCAUAa -3' miRNA: 3'- caaAUCGCU----UGUgUAUCGGG-UGUGUa -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 103905 | 0.69 | 0.98782 |
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Target: 5'- --gUAGCGAu--CGUAGCCCGC-CAa -3' miRNA: 3'- caaAUCGCUuguGUAUCGGGUGuGUa -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 80422 | 0.7 | 0.977658 |
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Target: 5'- ----uGCGGugACAuUGGCCCACGCc- -3' miRNA: 3'- caaauCGCUugUGU-AUCGGGUGUGua -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 17288 | 0.72 | 0.935717 |
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Target: 5'- uGUUUAGUGggUACAUucguaGGUCUACACGg -3' miRNA: 3'- -CAAAUCGCuuGUGUA-----UCGGGUGUGUa -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 161116 | 0.74 | 0.899508 |
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Target: 5'- uGUUUGGCGAACGgAUccguCCCGCACAa -3' miRNA: 3'- -CAAAUCGCUUGUgUAuc--GGGUGUGUa -5' |
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| 8732 | 5' | -48.6 | NC_002331.1 | + | 8137 | 1.05 | 0.023054 |
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Target: 5'- gGUUUAGCGAACACAUAGCCCACACAUc -3' miRNA: 3'- -CAAAUCGCUUGUGUAUCGGGUGUGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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