Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8733 | 3' | -45.4 | NC_002331.1 | + | 147385 | 0.66 | 0.99999 |
Target: 5'- aUCAAAGGCGaauguucUGCUGA-AACGUUGa -3' miRNA: 3'- aAGUUUUCGCga-----AUGACUaUUGCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 104118 | 0.66 | 0.999986 |
Target: 5'- aUCGu-AGCGCUU-CUGA-AACGCUu -3' miRNA: 3'- aAGUuuUCGCGAAuGACUaUUGCGAu -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 139219 | 0.66 | 0.999974 |
Target: 5'- -gCAAGAGUGCgUUAUUGAaauCGCUAc -3' miRNA: 3'- aaGUUUUCGCG-AAUGACUauuGCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 168718 | 0.66 | 0.999964 |
Target: 5'- cUCAGAu-CGCUUugUGAcAugGCUAg -3' miRNA: 3'- aAGUUUucGCGAAugACUaUugCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 6131 | 0.67 | 0.999882 |
Target: 5'- aUUCAGAuacgcGGCGCUUcaaACUGAUAcaaauaACGUUGu -3' miRNA: 3'- -AAGUUU-----UCGCGAA---UGACUAU------UGCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 103923 | 0.68 | 0.999574 |
Target: 5'- uUUCAGAAGCGC-UAC-GAUGuuaguGCGCUc -3' miRNA: 3'- -AAGUUUUCGCGaAUGaCUAU-----UGCGAu -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 34764 | 0.7 | 0.998433 |
Target: 5'- uUUCAAGuguGGCGCga--UGAUGACGCa- -3' miRNA: 3'- -AAGUUU---UCGCGaaugACUAUUGCGau -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 25513 | 0.7 | 0.997701 |
Target: 5'- cUCAAGAuaaCGCUaucagUACUGAUAGCGUUAu -3' miRNA: 3'- aAGUUUUc--GCGA-----AUGACUAUUGCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 67422 | 0.7 | 0.997239 |
Target: 5'- aUUCAAAcAGCuGCUugcucaaaUGCUGGUAGCGCa- -3' miRNA: 3'- -AAGUUU-UCG-CGA--------AUGACUAUUGCGau -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 109730 | 0.75 | 0.962865 |
Target: 5'- -aCAGAGGCGC-UGCUGGUAAaauUGCUGg -3' miRNA: 3'- aaGUUUUCGCGaAUGACUAUU---GCGAU- -5' |
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8733 | 3' | -45.4 | NC_002331.1 | + | 25284 | 1.05 | 0.045622 |
Target: 5'- uUUCAAAAGCGCUUACUGAUAACGCUAu -3' miRNA: 3'- -AAGUUUUCGCGAAUGACUAUUGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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