Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8735 | 3' | -51.2 | NC_002331.1 | + | 39339 | 0.66 | 0.992755 |
Target: 5'- --aGUGUG-AACa-GACGCUGGCugAu -3' miRNA: 3'- uuaCACAUgUUGagCUGCGACCGugU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 46870 | 0.66 | 0.991663 |
Target: 5'- ----cGUGCaAGCUCGACGCUGauuauuauguucGCACc -3' miRNA: 3'- uuacaCAUG-UUGAGCUGCGAC------------CGUGu -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 164976 | 0.66 | 0.991547 |
Target: 5'- uAAUGUuUACGAUcuuacugUCGACuGUUGGCACGu -3' miRNA: 3'- -UUACAcAUGUUG-------AGCUG-CGACCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 43737 | 0.66 | 0.98909 |
Target: 5'- --cGUGUAUAGaUUGuCGCUGaGCACAa -3' miRNA: 3'- uuaCACAUGUUgAGCuGCGAC-CGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 62325 | 0.66 | 0.98909 |
Target: 5'- -----uUGCGACUCGGCGUUGGuCugAu -3' miRNA: 3'- uuacacAUGUUGAGCUGCGACC-GugU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 43829 | 0.67 | 0.985938 |
Target: 5'- --aGUGggcauUGCGACUUGGCGUcgacGGCGCAa -3' miRNA: 3'- uuaCAC-----AUGUUGAGCUGCGa---CCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 153269 | 0.67 | 0.984121 |
Target: 5'- gAGUGUGUGCcgUUCGuCGaCUGGC-CGg -3' miRNA: 3'- -UUACACAUGuuGAGCuGC-GACCGuGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 48190 | 0.67 | 0.97996 |
Target: 5'- --cGUGUGCAAaaacaCGACGC-GGUACGu -3' miRNA: 3'- uuaCACAUGUUga---GCUGCGaCCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 66547 | 0.69 | 0.955073 |
Target: 5'- ----cGUACAGaacCGACGCgGGCACAg -3' miRNA: 3'- uuacaCAUGUUga-GCUGCGaCCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 98976 | 0.7 | 0.931789 |
Target: 5'- --cGUGUaccggaacaGCGGCUCGGcCGC-GGCACAa -3' miRNA: 3'- uuaCACA---------UGUUGAGCU-GCGaCCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 177256 | 0.7 | 0.920683 |
Target: 5'- aAAUGUGaauauauaacUACAAUUU-ACGCUGGCACGa -3' miRNA: 3'- -UUACAC----------AUGUUGAGcUGCGACCGUGU- -5' |
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8735 | 3' | -51.2 | NC_002331.1 | + | 67288 | 1.07 | 0.010994 |
Target: 5'- cAAUGUGUACAACUCGACGCUGGCACAg -3' miRNA: 3'- -UUACACAUGUUGAGCUGCGACCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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