Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8737 | 3' | -53.4 | NC_002331.1 | + | 151902 | 0.66 | 0.975118 |
Target: 5'- cCAACUGAUgG-ACGCG-GCGGUGcGGCa -3' miRNA: 3'- -GUUGACUGgCaUGCGCaUGCCGU-CUG- -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 107760 | 0.67 | 0.966504 |
Target: 5'- ---gUGACCGUACGCcaggggaugGUACGGaauuGGCu -3' miRNA: 3'- guugACUGGCAUGCG---------CAUGCCgu--CUG- -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 158501 | 0.67 | 0.966504 |
Target: 5'- -cGCUGGCCGUAUGUucgAUGGguGGa -3' miRNA: 3'- guUGACUGGCAUGCGca-UGCCguCUg -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 45175 | 0.67 | 0.966504 |
Target: 5'- aCAGCggaUGACCGUucuCGuCGUACGGCu--- -3' miRNA: 3'- -GUUG---ACUGGCAu--GC-GCAUGCCGucug -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 107291 | 0.69 | 0.899323 |
Target: 5'- aCAAUUGuucAUCGUugGU--ACGGCAGACg -3' miRNA: 3'- -GUUGAC---UGGCAugCGcaUGCCGUCUG- -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 97387 | 0.71 | 0.823717 |
Target: 5'- aGGC--GCCGUACGUuUACGGCGGAUc -3' miRNA: 3'- gUUGacUGGCAUGCGcAUGCCGUCUG- -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 145742 | 0.74 | 0.660008 |
Target: 5'- --uCUG-CCGUACGCGUACGGuCAGu- -3' miRNA: 3'- guuGACuGGCAUGCGCAUGCC-GUCug -5' |
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8737 | 3' | -53.4 | NC_002331.1 | + | 145539 | 1.09 | 0.006086 |
Target: 5'- aCAACUGACCGUACGCGUACGGCAGACc -3' miRNA: 3'- -GUUGACUGGCAUGCGCAUGCCGUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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