Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8738 | 3' | -51 | NC_002331.1 | + | 152104 | 0.66 | 0.995587 |
Target: 5'- ----cUGACgc-UGGCgGUCCAGGcGCa -3' miRNA: 3'- gugaaACUGauaACCGgCAGGUCC-UG- -5' |
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8738 | 3' | -51 | NC_002331.1 | + | 80725 | 0.67 | 0.990886 |
Target: 5'- cCACguuUUGAUauUGUUGGUCGgCCAGGcACu -3' miRNA: 3'- -GUGa--AACUG--AUAACCGGCaGGUCC-UG- -5' |
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8738 | 3' | -51 | NC_002331.1 | + | 40941 | 0.67 | 0.98959 |
Target: 5'- ----cUGAUagaUGUUGGCgGUCCAGGuCu -3' miRNA: 3'- gugaaACUG---AUAACCGgCAGGUCCuG- -5' |
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8738 | 3' | -51 | NC_002331.1 | + | 153652 | 0.71 | 0.911699 |
Target: 5'- uGCUU--ACUAUgGGCCGUUCuGGACg -3' miRNA: 3'- gUGAAacUGAUAaCCGGCAGGuCCUG- -5' |
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8738 | 3' | -51 | NC_002331.1 | + | 151863 | 1.1 | 0.008686 |
Target: 5'- aCACUUUGACUAUUGGCCGUCCAGGACc -3' miRNA: 3'- -GUGAAACUGAUAACCGGCAGGUCCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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