Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8738 | 5' | -61.4 | NC_002331.1 | + | 145753 | 0.66 | 0.682807 |
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Target: 5'- aUGCGCaUGGGuCUGCCGuacGCGUaCGGUCa -3' miRNA: 3'- gACGCGgACCU-GGCGGU---CGCA-GUCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 66457 | 0.67 | 0.642837 |
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Target: 5'- -aGCGCUUGGucaaccuGCUGCCcacGCGgcaaUCGGCCg -3' miRNA: 3'- gaCGCGGACC-------UGGCGGu--CGC----AGUCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 160287 | 0.68 | 0.594933 |
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Target: 5'- gUGCGCaCUGugucGACCGCCu-UGUUGGCCa -3' miRNA: 3'- gACGCG-GAC----CUGGCGGucGCAGUCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 99327 | 0.68 | 0.594933 |
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Target: 5'- uUGuCGCCauaUGGAgUGCUGGUguuGUCAGCCg -3' miRNA: 3'- gAC-GCGG---ACCUgGCGGUCG---CAGUCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 148613 | 0.7 | 0.481425 |
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Target: 5'- aUGUucaGCa-GGuCCGCCuGCGUCGGCCu -3' miRNA: 3'- gACG---CGgaCCuGGCGGuCGCAGUCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 168763 | 0.75 | 0.250091 |
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Target: 5'- gCUGCGCC-GuGACCGCUAGaGUCuGCCu -3' miRNA: 3'- -GACGCGGaC-CUGGCGGUCgCAGuCGG- -5' |
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| 8738 | 5' | -61.4 | NC_002331.1 | + | 151902 | 1.1 | 0.00087 |
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Target: 5'- gCUGCGCCUGGACCGCCAGCGUCAGCCu -3' miRNA: 3'- -GACGCGGACCUGGCGGUCGCAGUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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