Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8739 | 3' | -51.1 | NC_002331.1 | + | 51155 | 0.66 | 0.995734 |
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Target: 5'- aCGAUGGCGuUGAUGCGCUCUUUgGc- -3' miRNA: 3'- gGCUGCUGU-ACUGUGUGAGGAAgCua -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 150840 | 0.66 | 0.994242 |
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Target: 5'- uCCGuaAUGACAUuacGACACACUCaagCGAg -3' miRNA: 3'- -GGC--UGCUGUA---CUGUGUGAGgaaGCUa -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 160453 | 0.68 | 0.983654 |
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Target: 5'- -aGGCGAC---GCGCACUCCguuuggUCGAUg -3' miRNA: 3'- ggCUGCUGuacUGUGUGAGGa-----AGCUA- -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 101729 | 0.69 | 0.9658 |
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Target: 5'- gCGACGGCAcguuUGA-AUGCUCCUUUGGUg -3' miRNA: 3'- gGCUGCUGU----ACUgUGUGAGGAAGCUA- -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 154669 | 0.71 | 0.937322 |
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Target: 5'- gCCGuCGACAUGGCGCAUUUa-UCGGc -3' miRNA: 3'- -GGCuGCUGUACUGUGUGAGgaAGCUa -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 170983 | 0.71 | 0.921499 |
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Target: 5'- uUGACGACAUGGCAaaaAUUgCCUUUGAc -3' miRNA: 3'- gGCUGCUGUACUGUg--UGA-GGAAGCUa -5' |
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| 8739 | 3' | -51.1 | NC_002331.1 | + | 156196 | 1.08 | 0.01319 |
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Target: 5'- cCCGACGACAUGACACACUCCUUCGAUu -3' miRNA: 3'- -GGCUGCUGUACUGUGUGAGGAAGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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