Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 875 | 5' | -55.4 | NC_000898.1 | + | 102803 | 0.66 | 0.927479 |
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Target: 5'- --cAAGGCUCgucGCCGaGGUACCCAUg -3' miRNA: 3'- ccuUUCUGGGau-UGGCgCCGUGGGUG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 43048 | 0.67 | 0.904442 |
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Target: 5'- aGAGccAGACUgggAACCGCGGCAgcauUCCACc -3' miRNA: 3'- cCUU--UCUGGga-UUGGCGCCGU----GGGUG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 113186 | 0.67 | 0.890175 |
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Target: 5'- aGAAAGGCCCUAggaACCGUGGacuucaauaauaGCCUcagACa -3' miRNA: 3'- cCUUUCUGGGAU---UGGCGCCg-----------UGGG---UG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 1822 | 0.67 | 0.884714 |
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Target: 5'- gGGAccGACCgCagucGCgGCGGC-CCCGCg -3' miRNA: 3'- -CCUuuCUGG-Gau--UGgCGCCGuGGGUG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 154990 | 0.67 | 0.884714 |
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Target: 5'- gGGAccGACCgCagucGCgGCGGC-CCCGCg -3' miRNA: 3'- -CCUuuCUGG-Gau--UGgCGCCGuGGGUG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 16688 | 0.67 | 0.884714 |
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Target: 5'- uGGAAAGACCauuGCCGUGGaCACg--- -3' miRNA: 3'- -CCUUUCUGGgauUGGCGCC-GUGggug -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 142373 | 0.68 | 0.870442 |
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Target: 5'- gGGuGAGGACCCUAAaauucgcuaaCGgGGCAaCCGCa -3' miRNA: 3'- -CC-UUUCUGGGAUUg---------GCgCCGUgGGUG- -5' |
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| 875 | 5' | -55.4 | NC_000898.1 | + | 9021 | 0.73 | 0.588672 |
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Target: 5'- gGGggGGGCCCgGGCgCGgGGCGCaCCGg -3' miRNA: 3'- -CCuuUCUGGGaUUG-GCgCCGUG-GGUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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