miRNA: 3'- gACGaCUGACCgg-CGGUCACUcaUUC- -5'
miRNA: 3'- -GACGACUGACCGGCGGuc------ACUCauuc -5'
miRNA: 3'- gACGACUGACCG--------------GCGGUCACU-CAuuc -5'
miRNA: 3'- -GACGACUGACCGGCGGUCACUCAUUC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8764 | 5' | -55.2 | NC_002371.2 | + | 1747 | 0.68 | 0.472609 |
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Target: 5'- aUGCgGACUGGacaGCCAGUGAugAAGc -3' miRNA: 3'- gACGaCUGACCgg-CGGUCACUcaUUC- -5' |
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| 8764 | 5' | -55.2 | NC_002371.2 | + | 11975 | 0.7 | 0.393279 |
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Target: 5'- cCUGCUGAUUGGCUGCCucagccacUGAGc--- -3' miRNA: 3'- -GACGACUGACCGGCGGuc------ACUCauuc -5' |
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| 8764 | 5' | -55.2 | NC_002371.2 | + | 33303 | 0.71 | 0.328863 |
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Target: 5'- uUGCUGAUUGGCggauuaacaccaacCGCCAGUGAcGUu-- -3' miRNA: 3'- gACGACUGACCG--------------GCGGUCACU-CAuuc -5' |
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| 8764 | 5' | -55.2 | NC_002371.2 | + | 11719 | 1.08 | 0.000671 |
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Target: 5'- cCUGCUGACUGGCCGCCAGUGAGUAAGu -3' miRNA: 3'- -GACGACUGACCGGCGGUCACUCAUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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