Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8767 | 3' | -51.2 | NC_002371.2 | + | 25418 | 0.66 | 0.842958 |
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Target: 5'- uCGCcGCAGAG-GCGCUGCuGGAUg-- -3' miRNA: 3'- uGUGuCGUUUCuUGCGACG-CCUGuag -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 27249 | 0.66 | 0.832619 |
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Target: 5'- cUACAGCGGAGAugGagucaguUUGCGGucGCAUg -3' miRNA: 3'- uGUGUCGUUUCUugC-------GACGCC--UGUAg -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 16897 | 0.67 | 0.750848 |
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Target: 5'- ---uGGCAAcGAcCGCUGCGG-CAUCc -3' miRNA: 3'- ugugUCGUUuCUuGCGACGCCuGUAG- -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 11203 | 0.68 | 0.717197 |
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Target: 5'- -aGCAGCAAGagauauGGGCGCUaGCGG-UAUCa -3' miRNA: 3'- ugUGUCGUUU------CUUGCGA-CGCCuGUAG- -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 5498 | 0.72 | 0.453915 |
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Target: 5'- aGCACAGgaaguuuCGGAGAGCGCa--GGACAUCa -3' miRNA: 3'- -UGUGUC-------GUUUCUUGCGacgCCUGUAG- -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 4368 | 0.73 | 0.404008 |
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Target: 5'- cUACGGCAu----UGCUGCGGGCGUCc -3' miRNA: 3'- uGUGUCGUuucuuGCGACGCCUGUAG- -5' |
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| 8767 | 3' | -51.2 | NC_002371.2 | + | 26131 | 1.11 | 0.00114 |
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Target: 5'- gACACAGCAAAGAACGCUGCGGACAUCg -3' miRNA: 3'- -UGUGUCGUUUCUUGCGACGCCUGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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