Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8769 | 5' | -47.9 | NC_002371.2 | + | 3533 | 0.67 | 0.926692 |
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Target: 5'- aGGGCGuacuccuGCAGgGGCAGGCUGAacUGGcUAa -3' miRNA: 3'- -CCUGU-------CGUUgUCGUUUGACU--ACCuAU- -5' |
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| 8769 | 5' | -47.9 | NC_002371.2 | + | 22199 | 0.67 | 0.906425 |
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Target: 5'- cGGCGGCAugAGgaAAACUGGaaguuaaUGGAUGa -3' miRNA: 3'- cCUGUCGUugUCg-UUUGACU-------ACCUAU- -5' |
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| 8769 | 5' | -47.9 | NC_002371.2 | + | 11193 | 0.68 | 0.899772 |
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Target: 5'- aGGGCguGGCAGCAGCAAGagauAUGGGc- -3' miRNA: 3'- -CCUG--UCGUUGUCGUUUgac-UACCUau -5' |
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| 8769 | 5' | -47.9 | NC_002371.2 | + | 40232 | 0.7 | 0.809649 |
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Target: 5'- aGAUucuuuGUggUGGCGGACUGAUGGAa- -3' miRNA: 3'- cCUGu----CGuuGUCGUUUGACUACCUau -5' |
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| 8769 | 5' | -47.9 | NC_002371.2 | + | 6307 | 0.72 | 0.685838 |
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Target: 5'- ---gGGCAGCAGCu--UUGAUGGAUGa -3' miRNA: 3'- ccugUCGUUGUCGuuuGACUACCUAU- -5' |
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| 8769 | 5' | -47.9 | NC_002371.2 | + | 2172 | 1.1 | 0.002824 |
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Target: 5'- gGGACAGCAACAGCAAACUGAUGGAUAa -3' miRNA: 3'- -CCUGUCGUUGUCGUUUGACUACCUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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