Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8770 | 5' | -59.2 | NC_002371.2 | + | 8809 | 0.66 | 0.402088 |
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Target: 5'- cAUCGaauacgccuuuaUUGCCGUACCUG--UUGCCGCg -3' miRNA: 3'- -UAGC------------AGCGGCGUGGAUcaGACGGCGa -5' |
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| 8770 | 5' | -59.2 | NC_002371.2 | + | 36176 | 0.66 | 0.374941 |
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Target: 5'- cUCGUCGUCGUuUCUucUCUGCUGCUu -3' miRNA: 3'- uAGCAGCGGCGuGGAucAGACGGCGA- -5' |
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| 8770 | 5' | -59.2 | NC_002371.2 | + | 10968 | 0.67 | 0.366171 |
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Target: 5'- ---aUUGCCGCACCccaacGUCUGCgGCa -3' miRNA: 3'- uagcAGCGGCGUGGau---CAGACGgCGa -5' |
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| 8770 | 5' | -59.2 | NC_002371.2 | + | 7885 | 0.68 | 0.315795 |
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Target: 5'- uUUGUCGCCGCAcguuaucugguuuCCUucguacaugaAGUCgacGCCGCg -3' miRNA: 3'- uAGCAGCGGCGU-------------GGA----------UCAGa--CGGCGa -5' |
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| 8770 | 5' | -59.2 | NC_002371.2 | + | 5096 | 0.93 | 0.004416 |
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Target: 5'- uAUCGUCGCCGCAUCcAGUCUGCCGCUc -3' miRNA: 3'- -UAGCAGCGGCGUGGaUCAGACGGCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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