Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8772 | 3' | -53.1 | NC_002371.2 | + | 26828 | 1.11 | 0.000905 |
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Target: 5'- cCAGCACCUCCGAUACGGAAUUGCCGGa -3' miRNA: 3'- -GUCGUGGAGGCUAUGCCUUAACGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 26894 | 0.69 | 0.614083 |
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Target: 5'- cCGGCAaUUCCGuauCGGAggUGCUGGa -3' miRNA: 3'- -GUCGUgGAGGCuauGCCUuaACGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 26934 | 0.68 | 0.636728 |
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Target: 5'- cCAGCGCCUUuguaUGGUGCGGAugacccgcGCCGc -3' miRNA: 3'- -GUCGUGGAG----GCUAUGCCUuaa-----CGGCc -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 25360 | 0.68 | 0.658226 |
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Target: 5'- gCAGCGCCUCUGcgGCGauauaaauaaccuGGcgUGCaCGGu -3' miRNA: 3'- -GUCGUGGAGGCuaUGC-------------CUuaACG-GCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 9938 | 0.68 | 0.659356 |
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Target: 5'- aGGUGCgUCUGGUGCG--AUUGCUGGg -3' miRNA: 3'- gUCGUGgAGGCUAUGCcuUAACGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 23991 | 0.67 | 0.737085 |
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Target: 5'- aGGCAacUCUCCGGUAauucCGGGug-GCUGGa -3' miRNA: 3'- gUCGU--GGAGGCUAU----GCCUuaaCGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 4431 | 0.66 | 0.747841 |
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Target: 5'- gGGCAgCUUCGAcguauUGCGcuGAUUGCUGGg -3' miRNA: 3'- gUCGUgGAGGCU-----AUGCc-UUAACGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 33964 | 0.66 | 0.758473 |
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Target: 5'- gAGCGCCaUUCaGGUuguugagagaGGGAUUGCCGGc -3' miRNA: 3'- gUCGUGG-AGG-CUAug--------CCUUAACGGCC- -5' |
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| 8772 | 3' | -53.1 | NC_002371.2 | + | 16224 | 0.66 | 0.768967 |
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Target: 5'- --uUugCUCCaGAUGCaaGGAAUUGCCGc -3' miRNA: 3'- gucGugGAGG-CUAUG--CCUUAACGGCc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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