Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8792 | 3' | -54.4 | NC_002484.1 | + | 36928 | 0.66 | 0.770648 |
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Target: 5'- ---gAGCgCCUUGCcGccGAACGCCGa -3' miRNA: 3'- ccagUCGgGGAACGcUuaCUUGCGGC- -5' |
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| 8792 | 3' | -54.4 | NC_002484.1 | + | 50745 | 0.66 | 0.750154 |
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Target: 5'- cGGUCgccgggaccaGGCCCUgggcggUGCGGAUGAGagcgaguucaGCCa -3' miRNA: 3'- -CCAG----------UCGGGGa-----ACGCUUACUUg---------CGGc -5' |
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| 8792 | 3' | -54.4 | NC_002484.1 | + | 8603 | 0.68 | 0.686156 |
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Target: 5'- cGGUCAGCCCag-GCGGcaaAGCGuuGu -3' miRNA: 3'- -CCAGUCGGGgaaCGCUuacUUGCggC- -5' |
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| 8792 | 3' | -54.4 | NC_002484.1 | + | 17055 | 0.68 | 0.627982 |
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Target: 5'- uGGUCAGCCgCUcgacggugaguugcUGCGcgcUGuACGCCGu -3' miRNA: 3'- -CCAGUCGGgGA--------------ACGCuu-ACuUGCGGC- -5' |
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| 8792 | 3' | -54.4 | NC_002484.1 | + | 6300 | 0.7 | 0.512158 |
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Target: 5'- ---gAGCCCCUU-CGggUGGuCGCCGu -3' miRNA: 3'- ccagUCGGGGAAcGCuuACUuGCGGC- -5' |
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| 8792 | 3' | -54.4 | NC_002484.1 | + | 2317 | 1.1 | 0.001115 |
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Target: 5'- uGGUCAGCCCCUUGCGAAUGAACGCCGc -3' miRNA: 3'- -CCAGUCGGGGAACGCUUACUUGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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