Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8793 | 5' | -46.9 | NC_002484.1 | + | 28787 | 0.66 | 0.984678 |
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Target: 5'- --aGGAGGUGGGGAaUGAGCGAAgAa -3' miRNA: 3'- cugUCUUCAUCUCUaGUUCGCUUgUg -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 41531 | 0.67 | 0.98229 |
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Target: 5'- aGCGGAAGUGGAuucagcaugccggGAccaUCAAG-GAGCACu -3' miRNA: 3'- cUGUCUUCAUCU-------------CU---AGUUCgCUUGUG- -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 39927 | 0.67 | 0.977521 |
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Target: 5'- gGACAGGAGUugAGcAGAUgGAGCaAGCAg -3' miRNA: 3'- -CUGUCUUCA--UC-UCUAgUUCGcUUGUg -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 30544 | 0.67 | 0.977521 |
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Target: 5'- gGACAGGAGUugAGcAGAUgGAGCaAGCAa -3' miRNA: 3'- -CUGUCUUCA--UC-UCUAgUUCGcUUGUg -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 41426 | 0.68 | 0.97089 |
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Target: 5'- cGACGGcguAGcAGAGAUCAugaagcagcgccGCGAGCGCc -3' miRNA: 3'- -CUGUCu--UCaUCUCUAGUu-----------CGCUUGUG- -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 11543 | 0.68 | 0.956322 |
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Target: 5'- uGCGGAcGGUAGAGAcagugaGAGCGGcaGCGCg -3' miRNA: 3'- cUGUCU-UCAUCUCUag----UUCGCU--UGUG- -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 4463 | 0.69 | 0.946945 |
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Target: 5'- uGCAGcGG-AGAGGUCAggAGCGAcaGCGCc -3' miRNA: 3'- cUGUCuUCaUCUCUAGU--UCGCU--UGUG- -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 33810 | 0.69 | 0.946945 |
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Target: 5'- cGGCAGGAGaAGGGAUU--GUGAGCAa -3' miRNA: 3'- -CUGUCUUCaUCUCUAGuuCGCUUGUg -5' |
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| 8793 | 5' | -46.9 | NC_002484.1 | + | 2534 | 1.12 | 0.00465 |
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Target: 5'- gGACAGAAGUAGAGAUCAAGCGAACACa -3' miRNA: 3'- -CUGUCUUCAUCUCUAGUUCGCUUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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