Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 8794 | 3' | -56.2 | NC_002484.1 | + | 2686 | 1.06 | 0.001046 |
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Target: 5'- aAAGCAGCAUCGAUGCGACCAUGGCCUg -3' miRNA: 3'- -UUCGUCGUAGCUACGCUGGUACCGGA- -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 4033 | 0.7 | 0.392917 |
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Target: 5'- aAGGCGGCGcggcCGAUGCGgauuucaucccccGCCAUGGCg- -3' miRNA: 3'- -UUCGUCGUa---GCUACGC-------------UGGUACCGga -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 7922 | 0.66 | 0.631406 |
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Target: 5'- -cGCAuuuaaCAUCGAuguuugaauagaguUGCGGCCAUGGCg- -3' miRNA: 3'- uuCGUc----GUAGCU--------------ACGCUGGUACCGga -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 11418 | 0.66 | 0.623579 |
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Target: 5'- cGGCGGCAUCcAUGcCGGCCAcgaaGCCg -3' miRNA: 3'- uUCGUCGUAGcUAC-GCUGGUac--CGGa -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 32706 | 0.67 | 0.546059 |
|
Target: 5'- uGAGCuGCG-CGAUGCG-CUGcUGGCCUu -3' miRNA: 3'- -UUCGuCGUaGCUACGCuGGU-ACCGGA- -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 39379 | 0.67 | 0.524413 |
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Target: 5'- aGAGCGGCGcUGGUGUcacguuugcaauGGCCGUGGCg- -3' miRNA: 3'- -UUCGUCGUaGCUACG------------CUGGUACCGga -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 41286 | 0.68 | 0.482176 |
|
Target: 5'- cGGGCGGCAggacgUCGAUGCaGACgAcUGGCUg -3' miRNA: 3'- -UUCGUCGU-----AGCUACG-CUGgU-ACCGGa -5' |
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| 8794 | 3' | -56.2 | NC_002484.1 | + | 56078 | 0.66 | 0.620226 |
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Target: 5'- -uGUAGcCAUCucgcuugagGAUGCGcucucgcuuacgacGCCAUGGCCUg -3' miRNA: 3'- uuCGUC-GUAG---------CUACGC--------------UGGUACCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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