miRNA: 3'- -CGUUgACUCGU----AGCUGGCCUaACUAC- -5'
miRNA: 3'- cgUUGa-CUCGUa------GCUGGCCUa-ACUAC- -5'
miRNA: 3'- -CGUUG-ACUCGUAGCUGGCCUaacuac -5'
miRNA: 3'- -CGUUGACUCGUAGCUGGCCUAACUAC- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8795 | 5' | -49.4 | NC_002484.1 | + | 43990 | 0.66 | 0.955418 |
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Target: 5'- cGCGGaUGGGCAuucgUUGACUGGG-UGGUGa -3' miRNA: 3'- -CGUUgACUCGU----AGCUGGCCUaACUAC- -5' |
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| 8795 | 5' | -49.4 | NC_002484.1 | + | 33155 | 0.68 | 0.918015 |
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Target: 5'- -aAGCcaGAGCAggcggagCGGCCGGAggUGGUGg -3' miRNA: 3'- cgUUGa-CUCGUa------GCUGGCCUa-ACUAC- -5' |
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| 8795 | 5' | -49.4 | NC_002484.1 | + | 42981 | 0.76 | 0.502271 |
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Target: 5'- uGCAGCaUGAGCAUUGACUGGAg----- -3' miRNA: 3'- -CGUUG-ACUCGUAGCUGGCCUaacuac -5' |
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| 8795 | 5' | -49.4 | NC_002484.1 | + | 3149 | 1.11 | 0.003187 |
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Target: 5'- aGCAACUGAGCAUCGACCGGAUUGAUGc -3' miRNA: 3'- -CGUUGACUCGUAGCUGGCCUAACUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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