Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8796 | 5' | -60.8 | NC_002484.1 | + | 3693 | 1.11 | 0.000219 |
Target: 5'- gCGCCAGGUCGCACUGGACGCCCGAGCg -3' miRNA: 3'- -GCGGUCCAGCGUGACCUGCGGGCUCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 19516 | 0.71 | 0.212269 |
Target: 5'- aGCCAGGUCugGCGCaGGgcaaaugucaGCGUgCGAGCg -3' miRNA: 3'- gCGGUCCAG--CGUGaCC----------UGCGgGCUCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 40526 | 0.71 | 0.22896 |
Target: 5'- gGCuCuGGUCGCGCUGGAUGgCUGccGCa -3' miRNA: 3'- gCG-GuCCAGCGUGACCUGCgGGCu-CG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 49028 | 0.7 | 0.259231 |
Target: 5'- uCGCgUAGGUCGCugagcACcGGGCGCuuauCCGGGCg -3' miRNA: 3'- -GCG-GUCCAGCG-----UGaCCUGCG----GGCUCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 50750 | 0.69 | 0.299775 |
Target: 5'- uGCCucGGUCGC-CgGGACcagGCCCuGGGCg -3' miRNA: 3'- gCGGu-CCAGCGuGaCCUG---CGGG-CUCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 29547 | 0.68 | 0.321819 |
Target: 5'- uGCUcGG-CGCuCUGGACGCCCcgGAGa -3' miRNA: 3'- gCGGuCCaGCGuGACCUGCGGG--CUCg -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 52582 | 0.68 | 0.329431 |
Target: 5'- gCGCCAa---GCAUUGGAUGCCCGcugaagaucagGGCa -3' miRNA: 3'- -GCGGUccagCGUGACCUGCGGGC-----------UCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 32886 | 0.68 | 0.364488 |
Target: 5'- gGCCAgGGUCGCggagcuugagcugaaACUGGACcaGUCCGAcuacGCc -3' miRNA: 3'- gCGGU-CCAGCG---------------UGACCUG--CGGGCU----CG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 26516 | 0.67 | 0.412703 |
Target: 5'- gGCCAGGcCgGCACcucgaccgcGGGCGCUCGcacGGCa -3' miRNA: 3'- gCGGUCCaG-CGUGa--------CCUGCGGGC---UCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 19072 | 0.66 | 0.430852 |
Target: 5'- gCGCCA----GCGCUGGugGCCaGGGUg -3' miRNA: 3'- -GCGGUccagCGUGACCugCGGgCUCG- -5' |
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8796 | 5' | -60.8 | NC_002484.1 | + | 19309 | 0.66 | 0.468504 |
Target: 5'- cCGCCuGG-CGCAUgcacaUGcGGCGCCCcgGAGUu -3' miRNA: 3'- -GCGGuCCaGCGUG-----AC-CUGCGGG--CUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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