Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8798 | 5' | -55.1 | NC_002484.1 | + | 6094 | 1.15 | 0.000502 |
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Target: 5'- uGGCCUCUGCGACGUAUCUCUCCCACCa -3' miRNA: 3'- -CCGGAGACGCUGCAUAGAGAGGGUGG- -5' |
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| 8798 | 5' | -55.1 | NC_002484.1 | + | 11069 | 0.8 | 0.12683 |
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Target: 5'- uGGCUUCUGCGAUGg--UUCUgCCGCCa -3' miRNA: 3'- -CCGGAGACGCUGCauaGAGAgGGUGG- -5' |
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| 8798 | 5' | -55.1 | NC_002484.1 | + | 26791 | 0.69 | 0.551643 |
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Target: 5'- uGCaugUCUGgGACGUAgcugCUCUCCgaGCCg -3' miRNA: 3'- cCGg--AGACgCUGCAUa---GAGAGGg-UGG- -5' |
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| 8798 | 5' | -55.1 | NC_002484.1 | + | 4784 | 0.67 | 0.681644 |
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Target: 5'- aGGCuCUCUGCGAccauuuggcCGgcgGUCUucggugcuucCUCgCCGCCg -3' miRNA: 3'- -CCG-GAGACGCU---------GCa--UAGA----------GAG-GGUGG- -5' |
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| 8798 | 5' | -55.1 | NC_002484.1 | + | 6001 | 0.66 | 0.765589 |
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Target: 5'- uGGCCgcccUCUGCGGCGaggaGUUUCg-CCACUc -3' miRNA: 3'- -CCGG----AGACGCUGCa---UAGAGagGGUGG- -5' |
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| 8798 | 5' | -55.1 | NC_002484.1 | + | 24927 | 0.66 | 0.775594 |
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Target: 5'- aGGCCUgUGCucacuuCGgagucaacGUCUCUaccgCCCACCa -3' miRNA: 3'- -CCGGAgACGcu----GCa-------UAGAGA----GGGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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