Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8802 | 3' | -48 | NC_002484.1 | + | 31881 | 0.66 | 0.985187 |
Target: 5'- -aUCAUCA-GCGGGgcacuGGAGgGCGGCa -3' miRNA: 3'- cgAGUAGUcCGCUUau---CUUUgCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 20873 | 0.66 | 0.980841 |
Target: 5'- cGCUC-UCcggAGGUGAA-AGAAGagguUGCAGCa -3' miRNA: 3'- -CGAGuAG---UCCGCUUaUCUUU----GCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 32210 | 0.67 | 0.972627 |
Target: 5'- aGCUCGaagccgaguccCAGGCGcuaagGGAGgaagucGCGCAGCa -3' miRNA: 3'- -CGAGUa----------GUCCGCuua--UCUU------UGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 35359 | 0.67 | 0.969394 |
Target: 5'- -aUCAUCgcAGGCGAGcugccuUAcGAGcACGCGGCu -3' miRNA: 3'- cgAGUAG--UCCGCUU------AU-CUU-UGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 4867 | 0.67 | 0.965898 |
Target: 5'- aGC-CGUCgauggAGGUG-AUGGccGAGCGCGGCa -3' miRNA: 3'- -CGaGUAG-----UCCGCuUAUC--UUUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 43011 | 0.67 | 0.965898 |
Target: 5'- uGCacagUAUCAGGaacaGAacAUGGAgGAUGCAGCa -3' miRNA: 3'- -CGa---GUAGUCCg---CU--UAUCU-UUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 4060 | 0.67 | 0.965534 |
Target: 5'- -aUCAgcCAGGCGuuAUGGAucaucaggaaggcGGCGCGGCc -3' miRNA: 3'- cgAGUa-GUCCGCu-UAUCU-------------UUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 40379 | 0.67 | 0.958082 |
Target: 5'- cGCUgGUUcuGCGAAUGcGAagAAUGCGGCg -3' miRNA: 3'- -CGAgUAGucCGCUUAU-CU--UUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 52338 | 0.68 | 0.953747 |
Target: 5'- uUUCAUCAGGC-AGUccucuuuuucAGuucGCGCAGCu -3' miRNA: 3'- cGAGUAGUCCGcUUA----------UCuu-UGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 18565 | 0.68 | 0.94912 |
Target: 5'- aGUUCGUCGcGCGAAcGGAcgcCGCGGUa -3' miRNA: 3'- -CGAGUAGUcCGCUUaUCUuu-GCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 51114 | 0.68 | 0.944195 |
Target: 5'- cGC-CGUCAGGCGuAUGGAucagguggaaguGAC-CGGCc -3' miRNA: 3'- -CGaGUAGUCCGCuUAUCU------------UUGcGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 870 | 0.68 | 0.933437 |
Target: 5'- cGC-CAUCGgcGGCGAAUAGGuaaauGCcucguGCGGCa -3' miRNA: 3'- -CGaGUAGU--CCGCUUAUCUu----UG-----CGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 41836 | 0.69 | 0.9276 |
Target: 5'- uGCUCGUCgAGuGCGGAaucuGGACGCAGg -3' miRNA: 3'- -CGAGUAG-UC-CGCUUauc-UUUGCGUCg -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 44138 | 0.69 | 0.908251 |
Target: 5'- uGC-CAUCAcuuGGCGAGUu----CGCAGCg -3' miRNA: 3'- -CGaGUAGU---CCGCUUAucuuuGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 29601 | 0.7 | 0.893838 |
Target: 5'- gGCUCA--AGGCGGAgaacgAGGugcuGCGCAGg -3' miRNA: 3'- -CGAGUagUCCGCUUa----UCUu---UGCGUCg -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 7749 | 0.7 | 0.886188 |
Target: 5'- cGUUCGUCAgGGCGG--GGuuAgGCGGCu -3' miRNA: 3'- -CGAGUAGU-CCGCUuaUCuuUgCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 55837 | 0.73 | 0.746342 |
Target: 5'- aUUCAUCAGGaUGGAgggccagcaguccGGAACGCAGCa -3' miRNA: 3'- cGAGUAGUCC-GCUUau-----------CUUUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 38854 | 0.76 | 0.581616 |
Target: 5'- gGCUCA--AGGCGGAgaacGAGACGCuGCg -3' miRNA: 3'- -CGAGUagUCCGCUUau--CUUUGCGuCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 20039 | 0.76 | 0.581616 |
Target: 5'- cGUaUAUCAGGCcguGAcgguUAGGAACGCAGCa -3' miRNA: 3'- -CGaGUAGUCCG---CUu---AUCUUUGCGUCG- -5' |
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8802 | 3' | -48 | NC_002484.1 | + | 32632 | 0.78 | 0.481167 |
Target: 5'- aGUUCAUCGGGCGccuaucuGAAuuccucGCGCAGCg -3' miRNA: 3'- -CGAGUAGUCCGCuuau---CUU------UGCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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