Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8803 | 3' | -55.9 | NC_002484.1 | + | 45404 | 0.66 | 0.662912 |
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Target: 5'- uAUGgCGCGGUggCGGCaUUGGGUagGAGGUc -3' miRNA: 3'- -UACgGUGCCG--GUCGaAACCCA--CUUCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 41670 | 0.66 | 0.618321 |
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Target: 5'- -cGCCGCGcCCGGCaaggaggGGGUGugauGGCc -3' miRNA: 3'- uaCGGUGCcGGUCGaaa----CCCACu---UCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 52939 | 0.67 | 0.541105 |
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Target: 5'- aGUGCCGCGGCUugAGCUggcuGUGcAGCc -3' miRNA: 3'- -UACGGUGCCGG--UCGAaaccCACuUCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 35322 | 0.68 | 0.487948 |
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Target: 5'- aGUGCUAUGGa-GGcCUUUGGGUuucGGAGCg -3' miRNA: 3'- -UACGGUGCCggUC-GAAACCCA---CUUCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 25836 | 0.72 | 0.298137 |
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Target: 5'- -cGCCACcagguccuGGUUAGCcg-GGGUGAAGCc -3' miRNA: 3'- uaCGGUG--------CCGGUCGaaaCCCACUUCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 31567 | 0.73 | 0.255554 |
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Target: 5'- -aGCgCAUGGCCgauAGCUUUGGGUuGcAGCa -3' miRNA: 3'- uaCG-GUGCCGG---UCGAAACCCA-CuUCG- -5' |
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| 8803 | 3' | -55.9 | NC_002484.1 | + | 7486 | 1.09 | 0.000727 |
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Target: 5'- aAUGCCACGGCCAGCUUUGGGUGAAGCc -3' miRNA: 3'- -UACGGUGCCGGUCGAAACCCACUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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