Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8804 | 3' | -53.8 | NC_002484.1 | + | 38229 | 0.66 | 0.785928 |
Target: 5'- -gCCGGCCGG---CGCCGAUaucGCCGCu -3' miRNA: 3'- uaGGUCGGUUaggGUGGCUAa--UGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 44969 | 0.66 | 0.785928 |
Target: 5'- ----cGCCAGg-CCACCGAgccgACCGCu -3' miRNA: 3'- uagguCGGUUagGGUGGCUaa--UGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 24476 | 0.67 | 0.71235 |
Target: 5'- cAUCCgcAGCCGcgCUCGCCGuacuugcuCCGCu -3' miRNA: 3'- -UAGG--UCGGUuaGGGUGGCuaau----GGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 12847 | 0.67 | 0.71235 |
Target: 5'- gAUCCGGCCAgaucaaucgcaaAUCCUGcCCGAgcagUugCGUu -3' miRNA: 3'- -UAGGUCGGU------------UAGGGU-GGCUa---AugGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 48676 | 0.68 | 0.668247 |
Target: 5'- gAUUCuGCagggguAUUCCGCCGAcgGCCGCg -3' miRNA: 3'- -UAGGuCGgu----UAGGGUGGCUaaUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 13074 | 0.68 | 0.641468 |
Target: 5'- cAUCCAGCCAGUUgCuCUGAgccucauccugaGCCGCg -3' miRNA: 3'- -UAGGUCGGUUAGgGuGGCUaa----------UGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 17012 | 0.69 | 0.612406 |
Target: 5'- gAUCCgauAGCgCAG-CCCacgcggGCCGAUUGCUGCa -3' miRNA: 3'- -UAGG---UCG-GUUaGGG------UGGCUAAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 4109 | 0.69 | 0.600136 |
Target: 5'- cUCCAGCCAaucggcuAUCUCAcggagaucauuCCGAUUACCcaugGCg -3' miRNA: 3'- uAGGUCGGU-------UAGGGU-----------GGCUAAUGG----CG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 5806 | 0.69 | 0.590121 |
Target: 5'- -gCCGGCCuuUCUCACgCGAccGCCGUc -3' miRNA: 3'- uaGGUCGGuuAGGGUG-GCUaaUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 50268 | 0.69 | 0.579029 |
Target: 5'- uUCCGGUC-AUCCCgugagaaaACCGAgagcaACCGCa -3' miRNA: 3'- uAGGUCGGuUAGGG--------UGGCUaa---UGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 53838 | 0.7 | 0.546059 |
Target: 5'- -aCUGGCUAuaacgCCCACUGAacgUUGCCGCc -3' miRNA: 3'- uaGGUCGGUua---GGGUGGCU---AAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 15974 | 0.7 | 0.544969 |
Target: 5'- -gUCAGCCAcgcauggaucgucAUCCUgcgGCCGAUcggaUGCCGCu -3' miRNA: 3'- uaGGUCGGU-------------UAGGG---UGGCUA----AUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 50573 | 0.7 | 0.535197 |
Target: 5'- -gCUGGCCug-CCCACUGAgaaacuguUUGCCGCc -3' miRNA: 3'- uaGGUCGGuuaGGGUGGCU--------AAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 46677 | 0.7 | 0.524413 |
Target: 5'- cGUCUAGCCAucaGUCCCACUcca-ACUGCu -3' miRNA: 3'- -UAGGUCGGU---UAGGGUGGcuaaUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 11483 | 0.71 | 0.503102 |
Target: 5'- aAUCgCAGCC---CCCACCu-UUGCCGCa -3' miRNA: 3'- -UAG-GUCGGuuaGGGUGGcuAAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 17747 | 0.71 | 0.492588 |
Target: 5'- cGUCCAGCCGAucgaguUCCUcaagAUCGAgauCCGCa -3' miRNA: 3'- -UAGGUCGGUU------AGGG----UGGCUaauGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 55906 | 0.71 | 0.492588 |
Target: 5'- gAUCCGGCgGGUUCCAgCC--UUGCCGCc -3' miRNA: 3'- -UAGGUCGgUUAGGGU-GGcuAAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 52020 | 0.71 | 0.461681 |
Target: 5'- uGUUCGGCCAGUaCCUcgGCCuGAgacUUGCCGCa -3' miRNA: 3'- -UAGGUCGGUUA-GGG--UGG-CU---AAUGGCG- -5' |
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8804 | 3' | -53.8 | NC_002484.1 | + | 8389 | 1.11 | 0.000984 |
Target: 5'- gAUCCAGCCAAUCCCACCGAUUACCGCg -3' miRNA: 3'- -UAGGUCGGUUAGGGUGGCUAAUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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