Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8806 | 5' | -54.3 | NC_002484.1 | + | 29579 | 0.65 | 0.778496 |
Target: 5'- gCUGCGCAgguUCGCCGGCgaggcauaucaaGUGCUcgGCGCu -3' miRNA: 3'- -GGCGUGU---AGUGGUCGag----------UACGG--UGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 10330 | 0.66 | 0.772397 |
Target: 5'- aCGCcUGUC-CCGGCgugcgCAgGCCGCGCa -3' miRNA: 3'- gGCGuGUAGuGGUCGa----GUaCGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 31161 | 0.66 | 0.750646 |
Target: 5'- uCUGCGCAacggaggUCACCGGUUCAUcGUCgGCAa -3' miRNA: 3'- -GGCGUGU-------AGUGGUCGAGUA-CGG-UGUg -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 518 | 0.67 | 0.697962 |
Target: 5'- aCG-ACAUCGCUAGCguacUGUCGCGCa -3' miRNA: 3'- gGCgUGUAGUGGUCGagu-ACGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 27898 | 0.67 | 0.696866 |
Target: 5'- gCCgGCGCAUCcCCGGCaacCAUcaaccgagaacucGCCGCACu -3' miRNA: 3'- -GG-CGUGUAGuGGUCGa--GUA-------------CGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 26131 | 0.67 | 0.675928 |
Target: 5'- cUCGCgGCAUCcaacugcgGCCGGUa-GUGCCGCGCg -3' miRNA: 3'- -GGCG-UGUAG--------UGGUCGagUACGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 50937 | 0.68 | 0.642584 |
Target: 5'- -aGUACuUgGCCAGCcCAcgaaUGCCACGCa -3' miRNA: 3'- ggCGUGuAgUGGUCGaGU----ACGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 17062 | 0.68 | 0.631436 |
Target: 5'- cCCGCACugGUCAgCcGCUCGacggugaguUGCUGCGCg -3' miRNA: 3'- -GGCGUG--UAGUgGuCGAGU---------ACGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 30967 | 0.68 | 0.620288 |
Target: 5'- gCGCACcUCACCAGCUCcuucauUGgCugAUc -3' miRNA: 3'- gGCGUGuAGUGGUCGAGu-----ACgGugUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 36616 | 0.69 | 0.564899 |
Target: 5'- gCC-CugAUCGCCGGCggCAUGCCcggggaauGCGCc -3' miRNA: 3'- -GGcGugUAGUGGUCGa-GUACGG--------UGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 38347 | 0.69 | 0.564899 |
Target: 5'- aUGCGC--CGCCGGCUCGacGCCGCAg -3' miRNA: 3'- gGCGUGuaGUGGUCGAGUa-CGGUGUg -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 1623 | 0.7 | 0.532263 |
Target: 5'- cCCGCcuucucgaacaGCAUCACCAGggCG-GCCACGu -3' miRNA: 3'- -GGCG-----------UGUAGUGGUCgaGUaCGGUGUg -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 54300 | 0.71 | 0.469273 |
Target: 5'- aCCGCAC--CACuCGGCUCG-GCCAUAUu -3' miRNA: 3'- -GGCGUGuaGUG-GUCGAGUaCGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 38221 | 0.73 | 0.364855 |
Target: 5'- gCGCcgAUAUCGCCGcuguGCUCGgccUGCCGCGCg -3' miRNA: 3'- gGCG--UGUAGUGGU----CGAGU---ACGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 18244 | 0.73 | 0.35619 |
Target: 5'- gCCGggaACAUCGCCGGCaaggGCCACAUa -3' miRNA: 3'- -GGCg--UGUAGUGGUCGaguaCGGUGUG- -5' |
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8806 | 5' | -54.3 | NC_002484.1 | + | 11041 | 1.11 | 0.000882 |
Target: 5'- cCCGCACAUCACCAGCUCAUGCCACACc -3' miRNA: 3'- -GGCGUGUAGUGGUCGAGUACGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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