Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
8808 | 3' | -53.8 | NC_002484.1 | + | 18359 | 0.67 | 0.725269 |
Target: 5'- gGUAGAGcgcuggcgaguucaGCAGUGAccagucGAGCGGCu--CGCu -3' miRNA: 3'- -CAUCUC--------------UGUCACU------CUCGCCGuucGCG- -5' |
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8808 | 3' | -53.8 | NC_002484.1 | + | 38861 | 0.68 | 0.673596 |
Target: 5'- --cGGGACAGgcucaaggcgGAGAacgagacgcuGCGGgGAGCGCu -3' miRNA: 3'- cauCUCUGUCa---------CUCU----------CGCCgUUCGCG- -5' |
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8808 | 3' | -53.8 | NC_002484.1 | + | 31873 | 0.68 | 0.662446 |
Target: 5'- --cGGGGCAcugGAGGGCGGCAgcuucgauGGgGCa -3' miRNA: 3'- cauCUCUGUca-CUCUCGCCGU--------UCgCG- -5' |
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8808 | 3' | -53.8 | NC_002484.1 | + | 29216 | 0.7 | 0.549954 |
Target: 5'- gGUGGAGAaAGUGGGAcccucgacacgauGaCGGCGAcGCGCu -3' miRNA: 3'- -CAUCUCUgUCACUCU-------------C-GCCGUU-CGCG- -5' |
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8808 | 3' | -53.8 | NC_002484.1 | + | 11536 | 1.04 | 0.003127 |
Target: 5'- gGUAGAGACAGUGAGAGCGGC-AGCGCg -3' miRNA: 3'- -CAUCUCUGUCACUCUCGCCGuUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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