Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8815 | 5' | -53.5 | NC_002484.1 | + | 44481 | 0.66 | 0.799596 |
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Target: 5'- aCGUgGGCGGcgCCGu-CAGCUaccgagaCGGCCCu -3' miRNA: 3'- -GUAgCCGUCa-GGCuuGUCGA-------GUUGGG- -5' |
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| 8815 | 5' | -53.5 | NC_002484.1 | + | 52336 | 0.67 | 0.759376 |
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Target: 5'- uCAUCaGGCAGUCCucuuuuucaguucGcGCAGCUUGGCgCg -3' miRNA: 3'- -GUAG-CCGUCAGG-------------CuUGUCGAGUUGgG- -5' |
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| 8815 | 5' | -53.5 | NC_002484.1 | + | 16141 | 0.67 | 0.739482 |
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Target: 5'- uGUCGccGCGGU-CGAugAugcGCUCGGCCCa -3' miRNA: 3'- gUAGC--CGUCAgGCUugU---CGAGUUGGG- -5' |
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| 8815 | 5' | -53.5 | NC_002484.1 | + | 26623 | 0.68 | 0.728846 |
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Target: 5'- uGUUGGCAG-CC-AGCAGUUgGGCCUc -3' miRNA: 3'- gUAGCCGUCaGGcUUGUCGAgUUGGG- -5' |
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| 8815 | 5' | -53.5 | NC_002484.1 | + | 5418 | 0.68 | 0.728846 |
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Target: 5'- -cUCGGC-GUCCuuguuCAGCUCGaucagcGCCCa -3' miRNA: 3'- guAGCCGuCAGGcuu--GUCGAGU------UGGG- -5' |
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| 8815 | 5' | -53.5 | NC_002484.1 | + | 15621 | 1.13 | 0.000901 |
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Target: 5'- gCAUCGGCAGUCCGAACAGCUCAACCCg -3' miRNA: 3'- -GUAGCCGUCAGGCUUGUCGAGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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