Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8816 | 5' | -55.3 | NC_002484.1 | + | 788 | 0.66 | 0.741486 |
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Target: 5'- cUCGcGGAACCUGCGcuugccGGUGUCCcUCUuCa -3' miRNA: 3'- -AGC-UCUUGGGCGU------CCGCAGGuAGAuG- -5' |
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| 8816 | 5' | -55.3 | NC_002484.1 | + | 24853 | 0.67 | 0.66619 |
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Target: 5'- gCGguaAGGACCUGCGGGCGgcucgCCGcUCgGCg -3' miRNA: 3'- aGC---UCUUGGGCGUCCGCa----GGU-AGaUG- -5' |
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| 8816 | 5' | -55.3 | NC_002484.1 | + | 4223 | 0.69 | 0.541639 |
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Target: 5'- gUCGAcGGcuucgcGCCCGCcgauccaggucucGGCGUCCAUCUGu -3' miRNA: 3'- -AGCU-CU------UGGGCGu------------CCGCAGGUAGAUg -5' |
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| 8816 | 5' | -55.3 | NC_002484.1 | + | 51446 | 0.71 | 0.433392 |
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Target: 5'- gCGAcGACCCaGCAGGaCGaUCCGUCUGg -3' miRNA: 3'- aGCUcUUGGG-CGUCC-GC-AGGUAGAUg -5' |
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| 8816 | 5' | -55.3 | NC_002484.1 | + | 7015 | 0.73 | 0.319571 |
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Target: 5'- -aGAGGACCCGcCAGGUGUacaguuccaaCCGuUCUACg -3' miRNA: 3'- agCUCUUGGGC-GUCCGCA----------GGU-AGAUG- -5' |
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| 8816 | 5' | -55.3 | NC_002484.1 | + | 15809 | 1.09 | 0.000999 |
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Target: 5'- uUCGAGAACCCGCAGGCGUCCAUCUACc -3' miRNA: 3'- -AGCUCUUGGGCGUCCGCAGGUAGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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