Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8817 | 5' | -55.3 | NC_002484.1 | + | 29997 | 0.66 | 0.755499 |
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Target: 5'- uGCGGAgggUGGCuGAGGcgGCaacgCCGGGGCc -3' miRNA: 3'- -CGCCUg--GCCG-CUUUa-UGaa--GGCCCCG- -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 51857 | 0.67 | 0.703403 |
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Target: 5'- uGCGG-UCGGCGAuggcCUUCCGcgccGGCu -3' miRNA: 3'- -CGCCuGGCCGCUuuauGAAGGCc---CCG- -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 42859 | 0.68 | 0.649611 |
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Target: 5'- -aGGAa--GCGAAGUAUgggCCGGGGUg -3' miRNA: 3'- cgCCUggcCGCUUUAUGaa-GGCCCCG- -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 13490 | 0.69 | 0.57389 |
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Target: 5'- aGCGGugauGCCGGCagccGAAGcagccGCcgCCGGGGCc -3' miRNA: 3'- -CGCC----UGGCCG----CUUUa----UGaaGGCCCCG- -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 36620 | 0.72 | 0.385559 |
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Target: 5'- gGCGGcccugaucGCCGGCGgcAUGC--CCGGGGa -3' miRNA: 3'- -CGCC--------UGGCCGCuuUAUGaaGGCCCCg -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 4922 | 0.74 | 0.311665 |
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Target: 5'- uGCGGAgCGGC-AAcgACa-CCGGGGCg -3' miRNA: 3'- -CGCCUgGCCGcUUuaUGaaGGCCCCG- -5' |
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| 8817 | 5' | -55.3 | NC_002484.1 | + | 16406 | 1.15 | 0.000489 |
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Target: 5'- cGCGGACCGGCGAAAUACUUCCGGGGCg -3' miRNA: 3'- -CGCCUGGCCGCUUUAUGAAGGCCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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