Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8818 | 3' | -54 | NC_002484.1 | + | 26518 | 0.67 | 0.730653 |
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Target: 5'- cGGgCCAGGCCGGCAccucgACCGc--GGGc -3' miRNA: 3'- -CCaGGUCCGGCCGUaa---UGGUcaaCUC- -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 16965 | 0.69 | 0.598775 |
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Target: 5'- cGGUCUGcaacgcauuGGCCGGCGaaccgUUGCUguAGUUGAGc -3' miRNA: 3'- -CCAGGU---------CCGGCCGU-----AAUGG--UCAACUC- -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 18191 | 0.69 | 0.586632 |
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Target: 5'- cGUCUAGGCCGGUAUcgaacgaaUACuCAGUucuccgguaucgcUGAGc -3' miRNA: 3'- cCAGGUCCGGCCGUA--------AUG-GUCA-------------ACUC- -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 37224 | 0.7 | 0.554871 |
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Target: 5'- --aCCAGGCCGGCAUcgAUCAGa---- -3' miRNA: 3'- ccaGGUCCGGCCGUAa-UGGUCaacuc -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 29257 | 0.71 | 0.50144 |
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Target: 5'- -cUCCAGcCCGGCggUACCAGUaauccugccUGAGa -3' miRNA: 3'- ccAGGUCcGGCCGuaAUGGUCA---------ACUC- -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 12426 | 0.71 | 0.470481 |
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Target: 5'- --aCCAGuGCCGGCGUUACCAa-UGAa -3' miRNA: 3'- ccaGGUC-CGGCCGUAAUGGUcaACUc -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 3611 | 0.72 | 0.430782 |
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Target: 5'- uGGUUCuGGCUGGCugaACCAGUucaUGAGc -3' miRNA: 3'- -CCAGGuCCGGCCGuaaUGGUCA---ACUC- -5' |
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| 8818 | 3' | -54 | NC_002484.1 | + | 16627 | 1.1 | 0.001091 |
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Target: 5'- aGGUCCAGGCCGGCAUUACCAGUUGAGc -3' miRNA: 3'- -CCAGGUCCGGCCGUAAUGGUCAACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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