Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8821 | 5' | -55.6 | NC_002484.1 | + | 5019 | 0.66 | 0.629476 |
Target: 5'- -aGCgGCAUUguugaCGaAGCCGGUCUcGCg -3' miRNA: 3'- aaCGaCGUAAag---GCcUCGGCCAGA-CG- -5' |
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8821 | 5' | -55.6 | NC_002484.1 | + | 1177 | 0.66 | 0.674013 |
Target: 5'- -gGCUGUucucgugCCcccauGCCGGUCUGCa -3' miRNA: 3'- aaCGACGuaaa---GGccu--CGGCCAGACG- -5' |
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8821 | 5' | -55.6 | NC_002484.1 | + | 11786 | 0.7 | 0.399166 |
Target: 5'- -gGCcGCucagaUUCCGGAaCUGGUCUGCg -3' miRNA: 3'- aaCGaCGua---AAGGCCUcGGCCAGACG- -5' |
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8821 | 5' | -55.6 | NC_002484.1 | + | 26813 | 0.71 | 0.371946 |
Target: 5'- -cGCUGCAUg-CCGGAGaaucGUCUGCa -3' miRNA: 3'- aaCGACGUAaaGGCCUCggc-CAGACG- -5' |
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8821 | 5' | -55.6 | NC_002484.1 | + | 16984 | 1.1 | 0.000625 |
Target: 5'- aUUGCUGCAUUUCCGGAGCCGGUCUGCa -3' miRNA: 3'- -AACGACGUAAAGGCCUCGGCCAGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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