miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8824 3' -52 NC_002484.1 + 40352 0.66 0.867284
Target:  5'- -gGCGU-GUCCAUGAUCUcGCagaaCGACAa -3'
miRNA:   3'- cgUGCAgUAGGUACUAGA-CGc---GCUGU- -5'
8824 3' -52 NC_002484.1 + 26272 0.67 0.813868
Target:  5'- uGC-CGUCcccgAUCCAcagcCUGCGCGACAu -3'
miRNA:   3'- -CGuGCAG----UAGGUacuaGACGCGCUGU- -5'
8824 3' -52 NC_002484.1 + 12901 0.7 0.677036
Target:  5'- uGCugGaUC-UCCA-GGUCUGCGCG-CAg -3'
miRNA:   3'- -CGugC-AGuAGGUaCUAGACGCGCuGU- -5'
8824 3' -52 NC_002484.1 + 35050 0.72 0.57621
Target:  5'- aGUACGUCAUCgCGUacuacgcgGAUggauuccugcugCUGCGCGACAc -3'
miRNA:   3'- -CGUGCAGUAG-GUA--------CUA------------GACGCGCUGU- -5'
8824 3' -52 NC_002484.1 + 5136 0.79 0.242116
Target:  5'- -aGCGUCGUCCAgGAUCUG-GCGACu -3'
miRNA:   3'- cgUGCAGUAGGUaCUAGACgCGCUGu -5'
8824 3' -52 NC_002484.1 + 17832 1.12 0.001492
Target:  5'- aGCACGUCAUCCAUGAUCUGCGCGACAg -3'
miRNA:   3'- -CGUGCAGUAGGUACUAGACGCGCUGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.