Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8827 | 5' | -53.2 | NC_002484.1 | + | 49694 | 0.71 | 0.55863 |
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Target: 5'- uCCAGCGgGGagGCGGAgCGCu-UCGGCu -3' miRNA: 3'- -GGUCGCgCUa-UGCCUaGCGcuAGUCG- -5' |
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| 8827 | 5' | -53.2 | NC_002484.1 | + | 4847 | 0.72 | 0.537172 |
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Target: 5'- gCCgAGCGCGGcAUGGAUaCGCGAUgCgAGCc -3' miRNA: 3'- -GG-UCGCGCUaUGCCUA-GCGCUA-G-UCG- -5' |
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| 8827 | 5' | -53.2 | NC_002484.1 | + | 10930 | 0.72 | 0.526549 |
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Target: 5'- uCCAGCGCGccGCGac-CGgGAUCGGCc -3' miRNA: 3'- -GGUCGCGCuaUGCcuaGCgCUAGUCG- -5' |
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| 8827 | 5' | -53.2 | NC_002484.1 | + | 43086 | 0.72 | 0.505549 |
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Target: 5'- cCCGGaagGCGAUGCGGccgaaGCGAUCAagGCg -3' miRNA: 3'- -GGUCg--CGCUAUGCCuag--CGCUAGU--CG- -5' |
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| 8827 | 5' | -53.2 | NC_002484.1 | + | 50728 | 0.77 | 0.276545 |
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Target: 5'- cCCuGgGCGGUGCGGAUgagaGCGAguUCAGCc -3' miRNA: 3'- -GGuCgCGCUAUGCCUAg---CGCU--AGUCG- -5' |
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| 8827 | 5' | -53.2 | NC_002484.1 | + | 19591 | 1.13 | 0.001071 |
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Target: 5'- gCCAGCGCGAUACGGAUCGCGAUCAGCc -3' miRNA: 3'- -GGUCGCGCUAUGCCUAGCGCUAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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