Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8829 | 5' | -53.3 | NC_002484.1 | + | 10935 | 0.66 | 0.795873 |
Target: 5'- uUCCAUcCAGCGcgccGCGaCCGGGAucggccucGCCGGu -3' miRNA: 3'- -AGGUA-GUCGUu---CGCaGGCUCU--------UGGUC- -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 36717 | 0.66 | 0.785928 |
Target: 5'- gCCAaCGGCAGGCcgaCGAGcaGGCCAGg -3' miRNA: 3'- aGGUaGUCGUUCGcagGCUC--UUGGUC- -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 55380 | 0.67 | 0.765553 |
Target: 5'- aUCC-UCGGCGAGCG-CCu-GGAUCAGc -3' miRNA: 3'- -AGGuAGUCGUUCGCaGGcuCUUGGUC- -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 41899 | 0.68 | 0.723202 |
Target: 5'- --aGUCAuGCGugaaGGCGUCCGAGGAuaucgcCCAGg -3' miRNA: 3'- aggUAGU-CGU----UCGCAGGCUCUU------GGUC- -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 40589 | 0.68 | 0.701417 |
Target: 5'- aCgGUCAGCGaggggcuguugcGGCGUCUGAGcGACCu- -3' miRNA: 3'- aGgUAGUCGU------------UCGCAGGCUC-UUGGuc -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 55813 | 0.7 | 0.60125 |
Target: 5'- gUCCGgaacgCAGCAGGgGcUCGAGAACCu- -3' miRNA: 3'- -AGGUa----GUCGUUCgCaGGCUCUUGGuc -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 22938 | 0.72 | 0.441654 |
Target: 5'- uUCaCAUCAGacaaAGGCGUuuGAGGACCu- -3' miRNA: 3'- -AG-GUAGUCg---UUCGCAggCUCUUGGuc -5' |
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8829 | 5' | -53.3 | NC_002484.1 | + | 25006 | 1.09 | 0.0016 |
Target: 5'- aUCCAUCAGCAAGCGUCCGAGAACCAGc -3' miRNA: 3'- -AGGUAGUCGUUCGCAGGCUCUUGGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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