Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8830 | 3' | -55.9 | NC_002484.1 | + | 5302 | 0.66 | 0.676733 |
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Target: 5'- --gCUGCuCGGCGguuaccacuaCGCCAcucGGCGGCg -3' miRNA: 3'- gaaGACGuGCCGUag--------GCGGU---UCGCUG- -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 33761 | 0.66 | 0.643682 |
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Target: 5'- --aCUGCGCGaaAUCCGCCcGAGCa-- -3' miRNA: 3'- gaaGACGUGCcgUAGGCGG-UUCGcug -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 24843 | 0.66 | 0.63263 |
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Target: 5'- --cCUGCggGCGGCucgCCGCUcGGCGGg -3' miRNA: 3'- gaaGACG--UGCCGua-GGCGGuUCGCUg -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 30156 | 0.67 | 0.621578 |
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Target: 5'- --aCUGCAUGggcuauGCGUCaCGCCAucGGCGAa -3' miRNA: 3'- gaaGACGUGC------CGUAG-GCGGU--UCGCUg -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 4349 | 0.67 | 0.59951 |
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Target: 5'- --gCUuCGCGGCAuUCCGCUgcgagccaGGGCGGCa -3' miRNA: 3'- gaaGAcGUGCCGU-AGGCGG--------UUCGCUG- -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 32816 | 0.68 | 0.523597 |
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Target: 5'- ---aUGCGCGGCAUggCGaaaCAGGCGGCa -3' miRNA: 3'- gaagACGUGCCGUAg-GCg--GUUCGCUG- -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 40792 | 0.69 | 0.471681 |
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Target: 5'- cCUUC-GCGCaGCAgcUuuGCCGAGCGAa -3' miRNA: 3'- -GAAGaCGUGcCGU--AggCGGUUCGCUg -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 48675 | 0.69 | 0.461608 |
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Target: 5'- aUUCUGCAgGGguauUCCGCCGAcggccGCGAUc -3' miRNA: 3'- gAAGACGUgCCgu--AGGCGGUU-----CGCUG- -5' |
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| 8830 | 3' | -55.9 | NC_002484.1 | + | 25909 | 1.09 | 0.000785 |
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Target: 5'- aCUUCUGCACGGCAUCCGCCAAGCGACc -3' miRNA: 3'- -GAAGACGUGCCGUAGGCGGUUCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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