Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8832 | 3' | -50.7 | NC_002484.1 | + | 32248 | 0.66 | 0.930568 |
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Target: 5'- cGCCGUGaaGGAUcACgucaCGGAUGCCGAAUa -3' miRNA: 3'- -CGGCAC--CUUAcUG----GUCUACGGUUUGa -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 20334 | 0.66 | 0.9185 |
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Target: 5'- cGCUgaGUGGAAcuUGGCgAuauauggacGAUGCCAAACg -3' miRNA: 3'- -CGG--CACCUU--ACUGgU---------CUACGGUUUGa -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 30360 | 0.66 | 0.910021 |
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Target: 5'- aGCCGcgcggcucugagggUGGAGUuGCCGGAUGCUcuugugccggaGAACa -3' miRNA: 3'- -CGGC--------------ACCUUAcUGGUCUACGG-----------UUUGa -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 53986 | 0.69 | 0.812881 |
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Target: 5'- aGCCGcGGAAU----AGGUGCCGGACUa -3' miRNA: 3'- -CGGCaCCUUAcuggUCUACGGUUUGA- -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 42740 | 0.69 | 0.803066 |
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Target: 5'- cGCUGUGcGAGUGGCaCGGA--CCGAACa -3' miRNA: 3'- -CGGCAC-CUUACUG-GUCUacGGUUUGa -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 13589 | 0.71 | 0.696391 |
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Target: 5'- cGCCcuucUGGAGUaACCAGGUGCCGGuCUc -3' miRNA: 3'- -CGGc---ACCUUAcUGGUCUACGGUUuGA- -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 53889 | 0.73 | 0.582914 |
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Target: 5'- uGCCGcaugcgugauuUGGAGUGACCGG-UGCUGAAUc -3' miRNA: 3'- -CGGC-----------ACCUUACUGGUCuACGGUUUGa -5' |
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| 8832 | 3' | -50.7 | NC_002484.1 | + | 26763 | 1.1 | 0.002426 |
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Target: 5'- aGCCGUGGAAUGACCAGAUGCCAAACUu -3' miRNA: 3'- -CGGCACCUUACUGGUCUACGGUUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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