Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8834 | 5' | -56.6 | NC_002484.1 | + | 27875 | 1.1 | 0.000648 |
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Target: 5'- cAACCGAGAACUCGCCGCACUGUCCGCg -3' miRNA: 3'- -UUGGCUCUUGAGCGGCGUGACAGGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 24470 | 0.73 | 0.255139 |
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Target: 5'- cAGCCGc--GCUCGCCGUACUuGcUCCGCu -3' miRNA: 3'- -UUGGCucuUGAGCGGCGUGA-C-AGGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 29595 | 0.71 | 0.377371 |
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Target: 5'- aGGCgGAGAACgaggUGCUGCGCagGUUCGCc -3' miRNA: 3'- -UUGgCUCUUGa---GCGGCGUGa-CAGGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 17596 | 0.69 | 0.461536 |
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Target: 5'- uACCGAGA---CGCgGCuuuCUGUCUGCg -3' miRNA: 3'- uUGGCUCUugaGCGgCGu--GACAGGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 41686 | 0.69 | 0.481559 |
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Target: 5'- uGGCCGAGcuGCUCGCCGcCGC-GcCCGg -3' miRNA: 3'- -UUGGCUCu-UGAGCGGC-GUGaCaGGCg -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 38220 | 0.67 | 0.597811 |
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Target: 5'- cGCCGAua--UCGCCGCugUGcucggccugCCGCg -3' miRNA: 3'- uUGGCUcuugAGCGGCGugACa--------GGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 52004 | 0.66 | 0.641476 |
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Target: 5'- cGGCCuGAG-ACUUGCCGCAgcuuaUGcagCCGCu -3' miRNA: 3'- -UUGG-CUCuUGAGCGGCGUg----ACa--GGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 17885 | 0.66 | 0.641476 |
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Target: 5'- gGGCCGcAGAGaucaCGCCGCGCUuaauggucuucUCCGCa -3' miRNA: 3'- -UUGGC-UCUUga--GCGGCGUGAc----------AGGCG- -5' |
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| 8834 | 5' | -56.6 | NC_002484.1 | + | 40397 | 0.66 | 0.663296 |
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Target: 5'- gGGCCGcuGACUCGCaaaCGCUGguUCUGCg -3' miRNA: 3'- -UUGGCucUUGAGCGgc-GUGAC--AGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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