Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8836 | 5' | -66.8 | NC_002484.1 | + | 28610 | 0.66 | 0.218056 |
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Target: 5'- aGcCGGGCGCcacuagcucucCCUGagcuaaCCCGGCUggGCUc -3' miRNA: 3'- aC-GCCCGCG-----------GGAC------GGGCCGGuuCGGa -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 13811 | 0.66 | 0.212709 |
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Target: 5'- aUGCcuGGGCaaucGCCCgugaUGCCgGGCCGucGCCa -3' miRNA: 3'- -ACG--CCCG----CGGG----ACGGgCCGGUu-CGGa -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 14647 | 0.67 | 0.178363 |
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Target: 5'- -aCGGGCGCUCUGCC-GGCgcAGUCa -3' miRNA: 3'- acGCCCGCGGGACGGgCCGguUCGGa -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 36941 | 0.68 | 0.145218 |
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Target: 5'- aGCGGcugaagcugaGCGCCUUGCcgCCGaacGCCGAGCCc -3' miRNA: 3'- aCGCC----------CGCGGGACG--GGC---CGGUUCGGa -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 13956 | 0.69 | 0.12383 |
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Target: 5'- aGCGgugaaguGGCGCCgCUGCCUcGCCcuGCCUg -3' miRNA: 3'- aCGC-------CCGCGG-GACGGGcCGGuuCGGA- -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 39169 | 0.7 | 0.108805 |
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Target: 5'- aGCGGGCugauGCCUUGCauggaacGCCGAGCCUu -3' miRNA: 3'- aCGCCCG----CGGGACGggc----CGGUUCGGA- -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 49379 | 0.7 | 0.105956 |
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Target: 5'- uUGCGGGCGUCaCUGCUCGauccucccuUCAGGCCa -3' miRNA: 3'- -ACGCCCGCGG-GACGGGCc--------GGUUCGGa -5' |
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| 8836 | 5' | -66.8 | NC_002484.1 | + | 28517 | 1.06 | 0.000121 |
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Target: 5'- aUGCGGGCGCCCUGCCCGGCCAAGCCUc -3' miRNA: 3'- -ACGCCCGCGGGACGGGCCGGUUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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