Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 884 | 3' | -54 | NC_000898.1 | + | 2193 | 0.66 | 0.96187 |
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Target: 5'- cGGUCUGcgcGGCgACGuCGcGGCCGUCGcGCu -3' miRNA: 3'- -UCAGACa--CUG-UGC-GCaCUGGCAGU-CG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 155360 | 0.66 | 0.96187 |
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Target: 5'- cGGUCUGcgcGGCgACGuCGcGGCCGUCGcGCu -3' miRNA: 3'- -UCAGACa--CUG-UGC-GCaCUGGCAGU-CG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 31900 | 0.69 | 0.88344 |
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Target: 5'- cGGaUCUGUGACAauucCGauauucuaucUGACCGUCAGUu -3' miRNA: 3'- -UC-AGACACUGUgc--GC----------ACUGGCAGUCG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 774 | 0.72 | 0.756869 |
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Target: 5'- gGGUgUGgccGGCACGC---GCCGUCAGCg -3' miRNA: 3'- -UCAgACa--CUGUGCGcacUGGCAGUCG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 153942 | 0.72 | 0.756869 |
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Target: 5'- gGGUgUGgccGGCACGC---GCCGUCAGCg -3' miRNA: 3'- -UCAgACa--CUGUGCGcacUGGCAGUCG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 3417 | 0.78 | 0.404164 |
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Target: 5'- uGGgg-GUGAUAUGCgGUGACUGUCAGCg -3' miRNA: 3'- -UCagaCACUGUGCG-CACUGGCAGUCG- -5' |
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| 884 | 3' | -54 | NC_000898.1 | + | 156584 | 0.78 | 0.404164 |
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Target: 5'- uGGgg-GUGAUAUGCgGUGACUGUCAGCg -3' miRNA: 3'- -UCagaCACUGUGCG-CACUGGCAGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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