Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8843 | 5' | -53 | NC_002484.1 | + | 38238 | 0.66 | 0.837818 |
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Target: 5'- -cCugGuGAUGCCGGccgGCGCCGAuaucgccGCUg -3' miRNA: 3'- caGugC-CUACGGCUua-UGCGGUU-------CGA- -5' |
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| 8843 | 5' | -53 | NC_002484.1 | + | 26116 | 0.66 | 0.829604 |
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Target: 5'- --uGCGGccgguaGUGCCGc--GCGCCGAGCc -3' miRNA: 3'- cagUGCC------UACGGCuuaUGCGGUUCGa -5' |
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| 8843 | 5' | -53 | NC_002484.1 | + | 19465 | 0.66 | 0.800996 |
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Target: 5'- cUCACGGAcagaucGCCGAuu-UGCaCAAGCUc -3' miRNA: 3'- cAGUGCCUa-----CGGCUuauGCG-GUUCGA- -5' |
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| 8843 | 5' | -53 | NC_002484.1 | + | 16338 | 0.67 | 0.770728 |
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Target: 5'- uUCGCGGAUcGCau----CGCCGAGCUu -3' miRNA: 3'- cAGUGCCUA-CGgcuuauGCGGUUCGA- -5' |
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| 8843 | 5' | -53 | NC_002484.1 | + | 36604 | 0.78 | 0.215113 |
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Target: 5'- --gGCGGcAUGCCcgggGAAUGCGCCAAGCa -3' miRNA: 3'- cagUGCC-UACGG----CUUAUGCGGUUCGa -5' |
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| 8843 | 5' | -53 | NC_002484.1 | + | 32233 | 1.08 | 0.001985 |
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Target: 5'- cGUCACGGAUGCCGAAUACGCCAAGCUc -3' miRNA: 3'- -CAGUGCCUACGGCUUAUGCGGUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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