Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8845 | 5' | -56.5 | NC_002484.1 | + | 6718 | 0.66 | 0.623471 |
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Target: 5'- --cGGccgCCUUGCGCAGCGuuGCGa -3' miRNA: 3'- agaCUuaaGGAGCGCGUCGCgcCGCg -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 19313 | 0.67 | 0.612566 |
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Target: 5'- -gUGGccgCCUgGCGCAuGCacauGCGGCGCc -3' miRNA: 3'- agACUuaaGGAgCGCGU-CG----CGCCGCG- -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 6182 | 0.67 | 0.558465 |
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Target: 5'- cCUGAGUUCCUguaGUGCucgauGCGCGcCGUc -3' miRNA: 3'- aGACUUAAGGAg--CGCGu----CGCGCcGCG- -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 3039 | 0.69 | 0.485317 |
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Target: 5'- cCUGAAcUCCUCcCGCuGCGCaGGCu- -3' miRNA: 3'- aGACUUaAGGAGcGCGuCGCG-CCGcg -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 52323 | 0.69 | 0.445609 |
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Target: 5'- cUCUuu-UUCaguUCGCGCAGCuuGGCGCg -3' miRNA: 3'- -AGAcuuAAGg--AGCGCGUCGcgCCGCG- -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 3599 | 0.7 | 0.417051 |
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Target: 5'- gCUGAaccaGUUCaugaGCGCGGCGCGGaCGg -3' miRNA: 3'- aGACU----UAAGgag-CGCGUCGCGCC-GCg -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 14764 | 0.73 | 0.285891 |
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Target: 5'- --gGAAUUCCgacacguuguagaugUGcCGCAGCGCGGCGg -3' miRNA: 3'- agaCUUAAGGa--------------GC-GCGUCGCGCCGCg -5' |
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| 8845 | 5' | -56.5 | NC_002484.1 | + | 32616 | 1.12 | 0.000477 |
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Target: 5'- aUCUGAAUUCCUCGCGCAGCGCGGCGCu -3' miRNA: 3'- -AGACUUAAGGAGCGCGUCGCGCCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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