Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8846 | 5' | -53.2 | NC_002484.1 | + | 48980 | 0.66 | 0.777598 |
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Target: 5'- cCGCGGuugAGCUggcaGAGCgGGAGCaccGGGCa -3' miRNA: 3'- aGCGCC---UCGAa---CUCGaCUUUGa--CCUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 49683 | 0.67 | 0.74621 |
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Target: 5'- -gGCGGAGCgcuucGGCUcGGACUGGGu -3' miRNA: 3'- agCGCCUCGaac--UCGAcUUUGACCUg -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 52935 | 0.68 | 0.702719 |
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Target: 5'- cCGCG--GCUUGAGCUGGcugugcAGCcGGGCg -3' miRNA: 3'- aGCGCcuCGAACUCGACU------UUGaCCUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 29998 | 0.68 | 0.6693 |
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Target: 5'- cUGCGGAGggUG-GCUGAGGCggcaacgccgGGGCc -3' miRNA: 3'- aGCGCCUCgaACuCGACUUUGa---------CCUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 48744 | 0.7 | 0.557147 |
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Target: 5'- gUCGCGGAGCUUcaGGGU---AGCUGcGGCg -3' miRNA: 3'- -AGCGCCUCGAA--CUCGacuUUGAC-CUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 49488 | 0.72 | 0.450947 |
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Target: 5'- gUUGCGGAGCgcugUGAGCaccUGGAACgaacgccaGGGCu -3' miRNA: 3'- -AGCGCCUCGa---ACUCG---ACUUUGa-------CCUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 1425 | 0.73 | 0.402139 |
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Target: 5'- aCGCcGAacGCUUGGccuGCUGGAGCUGGGCc -3' miRNA: 3'- aGCGcCU--CGAACU---CGACUUUGACCUG- -5' |
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| 8846 | 5' | -53.2 | NC_002484.1 | + | 32879 | 1.09 | 0.001347 |
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Target: 5'- gUCGCGGAGCUUGAGCUGAAACUGGACc -3' miRNA: 3'- -AGCGCCUCGAACUCGACUUUGACCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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