Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8847 | 3' | -53.9 | NC_002484.1 | + | 35985 | 0.66 | 0.802159 |
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Target: 5'- cGCGgcgaGGACGACgacgGCAacgcGGCUGUGCc -3' miRNA: 3'- -UGUaaggCCUGCUGg---CGU----UCGACACG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 18376 | 0.67 | 0.730653 |
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Target: 5'- uCcgUCUGGGCG-CCGCAGGUaGaGCg -3' miRNA: 3'- uGuaAGGCCUGCuGGCGUUCGaCaCG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 37892 | 0.67 | 0.709144 |
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Target: 5'- cGCAUUaaugCCGGcagGCGGCUGCcAGCaugagGUGCa -3' miRNA: 3'- -UGUAA----GGCC---UGCUGGCGuUCGa----CACG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 26514 | 0.68 | 0.676332 |
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Target: 5'- cCAggCCGGcaccuCGACCGCGGGCgcucGCa -3' miRNA: 3'- uGUaaGGCCu----GCUGGCGUUCGaca-CG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 41762 | 0.68 | 0.676332 |
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Target: 5'- uCAgagUUCGucGACGACCGCGcGCUG-GCg -3' miRNA: 3'- uGUa--AGGC--CUGCUGGCGUuCGACaCG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 48999 | 0.69 | 0.587734 |
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Target: 5'- ---aUCCGGGCGAgacucacauCCGCGguugAGCUG-GCa -3' miRNA: 3'- uguaAGGCCUGCU---------GGCGU----UCGACaCG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 52947 | 0.7 | 0.533261 |
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Target: 5'- cGCGUUCCaGugccGCGGCUugaGCuGGCUGUGCa -3' miRNA: 3'- -UGUAAGGcC----UGCUGG---CGuUCGACACG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 48664 | 0.72 | 0.444457 |
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Target: 5'- gUAUUCCGccGACGGCCGCGaucaggcgauccauuAGCUG-GCu -3' miRNA: 3'- uGUAAGGC--CUGCUGGCGU---------------UCGACaCG- -5' |
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| 8847 | 3' | -53.9 | NC_002484.1 | + | 33467 | 1.11 | 0.001091 |
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Target: 5'- aACAUUCCGGACGACCGCAAGCUGUGCc -3' miRNA: 3'- -UGUAAGGCCUGCUGGCGUUCGACACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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