Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8849 | 3' | -57 | NC_002484.1 | + | 24756 | 0.66 | 0.624775 |
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Target: 5'- cGCCGGCucGAGG-GCcgGC-GUUCGGGAg -3' miRNA: 3'- -CGGCCG--CUUCaCGa-CGaCGAGCCUUg -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 51796 | 0.66 | 0.61383 |
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Target: 5'- gGCCGGgGAGcacacGcGCUGCcccagGCgcugCGGAACg -3' miRNA: 3'- -CGGCCgCUU-----CaCGACGa----CGa---GCCUUG- -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 41740 | 0.66 | 0.591996 |
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Target: 5'- cGCUGGCGAug-GCUGUUGCU---GACg -3' miRNA: 3'- -CGGCCGCUucaCGACGACGAgccUUG- -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 5325 | 0.67 | 0.570296 |
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Target: 5'- cGCCaGGCGGA-UGCggucgauucGCUGCUCGGc-- -3' miRNA: 3'- -CGG-CCGCUUcACGa--------CGACGAGCCuug -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 16949 | 0.68 | 0.465902 |
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Target: 5'- gGCCGGCGAAccGUUGCUGUaguuGAGCg -3' miRNA: 3'- -CGGCCGCUUcaCGACGACGagc-CUUG- -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 26825 | 0.69 | 0.436501 |
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Target: 5'- cGCCGGcCGGAucGCUGCaUGC-CGGAGa -3' miRNA: 3'- -CGGCC-GCUUcaCGACG-ACGaGCCUUg -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 27339 | 0.7 | 0.384616 |
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Target: 5'- cGCCGGCGuuccGCUGgcggauguucgggauCUGCUCGGAc- -3' miRNA: 3'- -CGGCCGCuucaCGAC---------------GACGAGCCUug -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 29567 | 0.73 | 0.251153 |
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Target: 5'- cGCCGGCGAggcauaucaAGUGCUcgGC-GCUCuGGACg -3' miRNA: 3'- -CGGCCGCU---------UCACGA--CGaCGAGcCUUG- -5' |
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| 8849 | 3' | -57 | NC_002484.1 | + | 34890 | 1.1 | 0.000525 |
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Target: 5'- gGCCGGCGAAGUGCUGCUGCUCGGAACc -3' miRNA: 3'- -CGGCCGCUUCACGACGACGAGCCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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