Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 8849 | 5' | -55.6 | NC_002484.1 | + | 3800 | 0.66 | 0.729343 |
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Target: 5'- ----aCCGGUCcaccAGUCGUAGCCAaUCGg -3' miRNA: 3'- ccuaaGGUCAG----UCGGCGUCGGUgAGC- -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 5966 | 0.67 | 0.664738 |
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Target: 5'- cGGAcucgUUCgCAGUCuucugcugugaGGCUGgGGCCACUUGc -3' miRNA: 3'- -CCU----AAG-GUCAG-----------UCGGCgUCGGUGAGC- -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 36440 | 0.67 | 0.653802 |
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Target: 5'- aGGuuaUCCAG-CGuGCCGCAGCCGaucCGg -3' miRNA: 3'- -CCua-AGGUCaGU-CGGCGUCGGUga-GC- -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 28433 | 0.67 | 0.653802 |
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Target: 5'- uGAUUUCAGUugaggCGGCCGCcGGCauuCUCGg -3' miRNA: 3'- cCUAAGGUCA-----GUCGGCG-UCGgu-GAGC- -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 55899 | 0.67 | 0.609957 |
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Target: 5'- cGGGUUCCAGcCuuGCCGCcuAGCUugUg- -3' miRNA: 3'- -CCUAAGGUCaGu-CGGCG--UCGGugAgc -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 13485 | 0.67 | 0.609957 |
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Target: 5'- uGAUgCCGG-CAGCCgaaGCAGCCGC-CGc -3' miRNA: 3'- cCUAaGGUCaGUCGG---CGUCGGUGaGC- -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 12898 | 0.74 | 0.282247 |
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Target: 5'- uGGAUcUCCAGgUCuGCgCGCAGUCGCUCc -3' miRNA: 3'- -CCUA-AGGUC-AGuCG-GCGUCGGUGAGc -5' |
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| 8849 | 5' | -55.6 | NC_002484.1 | + | 34856 | 1.12 | 0.000633 |
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Target: 5'- uGGAUUCCAGUCAGCCGCAGCCACUCGg -3' miRNA: 3'- -CCUAAGGUCAGUCGGCGUCGGUGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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