Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
8853 | 3' | -55.9 | NC_002484.1 | + | 40741 | 0.66 | 0.659036 |
Target: 5'- aGGAagucucagCACUGCgCGCAAGGGUGGuGg -3' miRNA: 3'- -UCUgca-----GUGAUGgGCGUUCCCGUCuC- -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 45787 | 0.66 | 0.648064 |
Target: 5'- ---aGUaCACUGCCCGCGgcgAGuGCGGAGa -3' miRNA: 3'- ucugCA-GUGAUGGGCGU---UCcCGUCUC- -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 33990 | 0.67 | 0.615102 |
Target: 5'- aAGGCGUUcugguCUACCCGgAAGuGGguGAu -3' miRNA: 3'- -UCUGCAGu----GAUGGGCgUUC-CCguCUc -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 4485 | 0.67 | 0.604132 |
Target: 5'- uGAUGUCuuguGCUGCCCGUAGugcaGCGGAGa -3' miRNA: 3'- uCUGCAG----UGAUGGGCGUUcc--CGUCUC- -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 27770 | 0.71 | 0.390319 |
Target: 5'- gGGGCGUgAUcguUGgCUGaCAAGGGCAGAGg -3' miRNA: 3'- -UCUGCAgUG---AUgGGC-GUUCCCGUCUC- -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 53960 | 0.71 | 0.372567 |
Target: 5'- cGGCGUCAC-GCCCGCAgagcaAGGaGCcGGGg -3' miRNA: 3'- uCUGCAGUGaUGGGCGU-----UCC-CGuCUC- -5' |
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8853 | 3' | -55.9 | NC_002484.1 | + | 37461 | 1.09 | 0.000852 |
Target: 5'- cAGACGUCACUACCCGCAAGGGCAGAGa -3' miRNA: 3'- -UCUGCAGUGAUGGGCGUUCCCGUCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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