miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
8853 3' -55.9 NC_002484.1 + 40741 0.66 0.659036
Target:  5'- aGGAagucucagCACUGCgCGCAAGGGUGGuGg -3'
miRNA:   3'- -UCUgca-----GUGAUGgGCGUUCCCGUCuC- -5'
8853 3' -55.9 NC_002484.1 + 45787 0.66 0.648064
Target:  5'- ---aGUaCACUGCCCGCGgcgAGuGCGGAGa -3'
miRNA:   3'- ucugCA-GUGAUGGGCGU---UCcCGUCUC- -5'
8853 3' -55.9 NC_002484.1 + 33990 0.67 0.615102
Target:  5'- aAGGCGUUcugguCUACCCGgAAGuGGguGAu -3'
miRNA:   3'- -UCUGCAGu----GAUGGGCgUUC-CCguCUc -5'
8853 3' -55.9 NC_002484.1 + 4485 0.67 0.604132
Target:  5'- uGAUGUCuuguGCUGCCCGUAGugcaGCGGAGa -3'
miRNA:   3'- uCUGCAG----UGAUGGGCGUUcc--CGUCUC- -5'
8853 3' -55.9 NC_002484.1 + 27770 0.71 0.390319
Target:  5'- gGGGCGUgAUcguUGgCUGaCAAGGGCAGAGg -3'
miRNA:   3'- -UCUGCAgUG---AUgGGC-GUUCCCGUCUC- -5'
8853 3' -55.9 NC_002484.1 + 53960 0.71 0.372567
Target:  5'- cGGCGUCAC-GCCCGCAgagcaAGGaGCcGGGg -3'
miRNA:   3'- uCUGCAGUGaUGGGCGU-----UCC-CGuCUC- -5'
8853 3' -55.9 NC_002484.1 + 37461 1.09 0.000852
Target:  5'- cAGACGUCACUACCCGCAAGGGCAGAGa -3'
miRNA:   3'- -UCUGCAGUGAUGGGCGUUCCCGUCUC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.